Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene
XU Chun-bo~1,MI Fu-gui~2,WANG-Yong~2(1.Grassland Research Institute of Chinese Academy of Agriculture Sciences,Huhhot,Inner Mongolia Autonomous Region 010010,China;2.Inner Mongolia Agriculture University,Huhhot,Inner Mongolia Autonomous Region 010019,China)
Plants transformed by P5CS gene(97#、19#、66#) and negatives were tested using several salt tolerance index such as proline content,leaf-water content,plasma membrane permeability and ratio of K~+/Na~+.The results show that the transgenic wheatgrass plants accumulated more proline than CK under salt-stress conditions,which proved that P5CSF129A had functioned in transgenic wheatgrass plants and had increased their salt-tolerance.The results also show that transgenic wheatgrass plants had nurtured greater salinity tolerance than CK.According to physiological target in synthetically evaluation,the result is 97#19#66#Negatives.
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