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《Acta Agrestia Sinica》 2007-02
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Study on the Verification of Alfalfa Cultivars by RAPD Molecular Markers

MAO Pei-sheng1,ZHANG Tao1,2,YANG Qing-chuan3 (1.Institute of Grassland Science,China Agriculture University,Beijing 100094,China;2.Department of Food Science,Tianjin Agriculture University,Tianjin 300384,China;3.Institute of Animal and Veterinary Science,CAAS,Beijing 100094,China)  
Alfalfa,a perennial legume forage,is important for the establishment of cultivated grassland and production of hay and silage.Superior cultivars of alfalfa have been distributed to large areas of northern China. Four alfalfa cultivars: Zhongmu No.1,Zhaodong,Gannong No.3,and Gongnong No.1 were selected as research materials.The present experiment screened primers which could verify cultivars by amplifying specific nucleotide sequence in DNA (mixed by 60 individuals) by RAPD-PCR.The results show that 120 primers were screened and four primers: S1,S78,S89,S115,could mark specific nucleotide sequence;electrophoresis bands were clear and specific.These different alfalfa cultivars could be identified by combination of four primers.The PCR amplified effect of RAPD in individual plants of each cultivar indicated that the rate of individuals with specific marker was high and steady.
【Key Words】： Alfalfa Verification of cultivar RAPD
【CateGory Index】： S541.9
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