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Methodological Study for Total Flavonoid Extraction from Alfalfa by Microwave Assistance

ZHANG Yong-mei,YAN Shi-juan,CAO Zhi-zhong,SHI Shang-li(Pratacultural College,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China)  
Medicago L.,an important medical and edible grass,is rich in natural bioactive substances.In order to make full use of flavonoids in M.sativa L.,the microwave-assisted flavonoid extraction process was studied by one-factor experiment of ethanol concentration,microwave power,ratio of solid to liquid,and processing time,and extraction times,respectively, and the orthogonal experiment based on the one-factor experiments.The results show that ratio of solid to liquid and microwave power were main factors and the others were minors.The parameters for optimized extraction process were ethanol concentration at 40%,ratio of solid to liquid at 1∶30,microwave power at 500 W,and radiation time at 60 seconds.The amount of flavonoids extracted from alfalfa was 4.88 mg/g under the optimized extraction process and similar to the theoretical content at 4.92 mg/g.
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