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《Acta Agrestia Sinica》 2008-03
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Genetic Diversity Analysis by SSR Marker of Fourteen Species of Caragana Fabr. in He-Xi Corridor Area of Gansu

GUO Qiang1,SHI Yong-jie2,WEI Zhen-wu1,YANG Zhan-hua1,LU Juan1,JIA Yan-qiong3 (1.Pratacultural College,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China;2.Lanzhou Institute of Animal Husbandry and Medicine,Chinese Academy of Agricultural Science,Lanzhou,Gansu Province 730050,China;3.Business School,Assumption University,Bangkok 10240,Thailand)  
The genetic diversity and relationship were investigated using 9 pairs of nSSR(nuclear simple sequence repeat) and 10 pairs of cpSSR(chloroplast simple sequence repeat) markers among 14 Species of Caragana Fabr.collected from Minqin Desert Botanical Garden of Gansu Province.Total 109 polymorphic loci were detected by 9 pairs of nSSR primers and the value of allelic PIC(polymorphism information content) ranged from 0.320 to 0.920,averaged 0.738 per primer.Total 92 polymorphic loci were detected by 10 pairs of cpSSR primers and the value of allelic PIC ranged from 0.512 to 0.865,averaged 0.671 per primer.The value of genetic similarity(GS) indexes among those 14 species varied from 0.60 to 0.92 and averaged 0.76.Cluster analysis classified those 14 Species into 5 groups and 6 sub-groups.The interspecific genetic relationship analyzed by nSSR and cpSSR was almost consistent with the result by traditional classification method.The results of molecular markers assay indicated that the Caragana Fabr.had a high level of genetic diversity which could be theoretical basis for the germplasm utilization and breeding of Caragana Fabr.
【Fund】: 世界银行贷款甘肃牧业发展项目-全球环境基金赠款(GEF)项目(052456-CHA)
【CateGory Index】: Q943
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