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《Acta Agrestia Sinica》 2008-06
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Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose

ZHAO Ying1,2,FU Hai-tian3,TIAN Wei-min1,JIANG Chang-shun1*(1.Key Laboratory for Tropical Crop Physiology,Ministry of Agriculture and Chinese Academy of Tropical Agricultural Science,Danzhou,Hainan Province 571737,China;2.College of Agronomy,Hainan University,Danzhou,Hainan Province 571737,China;3.Guangxi Subtropical Crops Research Institute,Nanning,Guangxi Province 530001,China)  
Leaves of anthracnose resistant cultivar Stylosanthes guianensis(Aubl.) Sw.cv.Mineirao and anthracnose sensitive cultivar S.guianensis cv.CIAT2312 were tested for activities of phenylalanine ammonialyase(PAL),polyphenol oxidase(PPO),and superoxide dismutase(SOD) after inoculation of Collectotrichum gloeosporioides(Penz.) Sacc.The results showed that the activities of the three enzymes were similar in Mineriao and CIAT2312.After inoculation,the activities of the three enzymes increased at first and declined afterward,the increment of Mineriao was more than CIAT2312.It was concluded that the variation of PAL,PPO,SOD activities in the inoculated leaves were closely correlated with the Stylosanthes cultivar resistance against anthracnose.PAL gene was cloned from Mineirao and its expression level was higher than CIAT2312 after inoculation by the test of semi-quantitative RT-PCR.We speculated that PAL gene had function in the anthracnose resistance of Stylosanthes.
【Fund】: 国家自然科学基金(No.30460087);; 华南热带农业大学科技基金(No.Rnd0407);; 热带作物栽培生理学重点实验室开放课题基金(KLOF0510)资助
【CateGory Index】: S541.9
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