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《Acta Agrestia Sinica》 2009-02
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Callus Induction and Plant Regeneration from Euphyllas of Bothriochloa ischaemum

DONG Kuan-hu,YU Na,CHENG Yu-hong (College of Animal Science and technology,Shanxi Agricultural University,Taigu,Shanxi Province 030801,China)  
The feasible hormone combinations were selected for callus induction and plantlet regeneration of Bothriochloa ischaemum(L.) Keng.using the 7-day old euphyllas of as explants,in order to build plamtlet regeneration system.The results show that the effect of KT was in evidence on callous induction,the interaction of KT and 2,4-D was superior than that of ABA and 2,4-D or 2,4-D only.The maximal callus induction rate reached 93.45% when the callus induction medium supplemented with 1.0 mg L-1 2,4-D and 0.5 mg L-1 KT.When the callus were transferred to the medium containing 0.1 mg L-1 6-BA and 0.04-0.06 mg L-1 NAA,the differentiation frequency of shoot was not significantly different.
【Fund】: 国家科技支撑计划项目(2007BAD56B01);; 农业部公益性行业(农业)科研专项(nyhyzx07-022)资助
【CateGory Index】: S543.9
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