Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Agrestia Sinica》 2009-06
Add to Favorite Get Latest Update

Analysis of Longdong Wild Alfalfa Based on Peroxidase Isozymes Zymogram

CHEN Li-qiang,SHI Shang-li*,WANG Cai-fen(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education Sino-U.S.Centers for Grazingland Ecosystem Sustainability.Lanzhou 730070,China)  
Longdong wild alfalfa(Medicago sativa L.cv.Longdong) is a rhizomatous perennial autotetraploid(2n=4x=32) legume forage found in the Longdong region of Gansu.It is considered to be Medicago sativa on the basis of its morphology,and has potential as forage because of its high branching ability,presence of rhizomes and its tolerance to trampling,cold and drought.but little is known about the genetic differences between Longdong wild alfalfa and cultivars.Peroxidase(POD) isozymes of Longdong wild alfalfa and other 38 alfalfa cultivars were analyzed using polyacrylamide gel electrophoresis.The genetic relationship among 39 cultivars was studied primarily based on cluster analysis and calculation of euclidean distance.The results showed that the POD isozymes zymograms of accessions had eleven isozyme bands which included four main ones,among which Longdong wild alfalfa had seven.39 alfalfa accessions were clustered into 6 groups using average linkage(between groups).Longdong wild alfalfa was clustered in an independent group.The Euclidean's distance between Longdong wild alfalfa and other tested materials varied from 0.748 to 1.622 with an average of 0.795 which were much more than between the cultivars which ranged from 0.533 to 1.029 with an average of 0.795,suggesting that the genetic distance between Longdong wild alfalfa and other 38 accessions was relatively wide.According to the genetic distance and cluster analysis,Longdong wild alfalfa can be regarded as distinct alfalfa germplasm,with the potential to broaden the genetic basis of cultivars developed by breeding programs.
【Fund】: “十一五”国家科技支撑计划(2006BAD01A19)、(2006BAD04A04-03)、(2007BAD52B06);; 农业部公益性行业(农业)科研专项(nyhyzx07-022)资助
【CateGory Index】: S541.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 SHA Wei, MENG Fan-ling, FENG Chang-jun(College of Life Science and Engineering, Qiqihaer University, Heilongjiang 161006);A Study on Isozymes of Alfalfa in Different Growth Period[J];Northern Horticulture;2007-01
2 ZHANG Xue-ting,SHI Shang-li*(Pratacultural College,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem of Ministry of Education; Sino-U.S.Centers for Grazingland Ecosystem Sustainability;Lanzhou,Gansu Province 730070,China);Genetic specificity in Native Medicago sativa L. cv. Longdong[J];Acta Agrestia Sinica;2009-03
3 WANG Ya-ling,SHI Shang-li,JIAO Liang(Pratacultural College of Gansu Agricultural University,Lanzhou 730070,China);Study on biological characteristics of Longdong wild alfalfa[J];Pratacultural Science;2008-01
4 SHENG Yunyan,LUAN Feishi,CHEN Kenong(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Genetic diversity of Chinese melon cultivars(Cucumis melo L.) based on simple sequence repeat markers[J];Journal of Northeast Agricultural University;2006-02
5 Gong Dajie (Department of Biology,Northwest Normal University 730070 Lanzhou PRC) Zhou Kaiya (Department of Biology,Nanjing Normal University 210097 Nanjing PRC);Isozyme and its application in systematics of reptiles[J];JOURNAL OF NORTHWEST NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1999-01
6 HUANG Jian-xun,ZHANG Kai,JIANG Liang-rong~*,HUANG Yu-min,WANG Hou-cong(School of Life Sciences,Xiamen University,Xiamen 361005,China);Genetic Diversity Analysis Based on SSR in Indica Varieties[J];Journal of Xiamen University(Natural Science);2006-01
7 Li Xiaolin (Department of Horticulture, Southwest Agricultural University, Chongqing 630716);ANALYSIS OF ESTERASE ISOZYMES IN MALUS MILL.[J];Journal of Southwest Agricultural University;1997-02
8 LIU Bo,WANG Li,CHEN Shu-ying,YANG Yan-qiong,HE Shi-ping,ZHAN Yan(Faculty of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China);Study on the Isozymes of 36 Different Pinellia ternate Populations[J];Journal of Yunnan Agricultural University;2008-01
9 XIE Ke_jun1,LI Yang_chun2,WU Tian_de3(1.Institute of Agricultural Bio-Enviroment,Zhejiang University,Hangzhou 310029;2.Faculty of Pratacultural,Gansu Agricultural University Lanzhou 730070;3.Animal Nutrition Institute of Gansu,Lanzhou730030):Grassland of China,No.2,2003,pp.30~33.;Analysis on Peroxidase Isozyme of 10 Species of Poa.[J];Grassland of China;2003-02
10 CHEN Chuan-jun,SHEN Yi-xin,ZHOU Jian-guo(College of Animal Science and Technology, Nanjing Agricultural University,Nanjing 210095,China);Zymogram Characteristics of Peroxidase Isozymes of Nannong Line Kentucky Bluegrass and Its Differences between Other Cultivars.[J];Grassland of China;2005-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Ke-nong1,JI Peng2,SHENG Yun-yan1(1.Horticulture Department,Northeast Agriculture University,Harbin,Heilongjiang 150030,China;2.Plant Technology Department,August First Land Reclamation University,Daqing,Heilongjiang 163319,China);Relationship Between Genetic Distance and Heterosis on Melon[J];Northern Horticulture;2008-05
2 JIA Shou\|Ju\ ZHANG Yong\|Pu\ CHEN Yan\|Le\ JIN Ai\|Hong ZHENG Xue\|Jiao\ JIANG Wei\|Peng (Department of Biological and Environmental Sciences,Wenzhou Normal College\ Wenzhou\ 325027,China);Comparative Studies on Isozymes of Eumeces chinensis[J];Chinese Journal of Zoology;2002-05
3 MA Yan-hong,YU Zhuo~*,ZHAO Xiao-jie,LIU Jie (Agronomy college, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, 010019,China);Isozyme Analysis of Chromosome-Doubling Plant of Triploid Hybrid of Elymus Canadensis and Hordeum Brevisubulatum[J];Acta Agrestia Sinica;2004-02
4 ZHANG Xue-ting,SHI Shang-li*(Pratacultural College,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem of Ministry of Education; Sino-U.S.Centers for Grazingland Ecosystem Sustainability;Lanzhou,Gansu Province 730070,China);Genetic specificity in Native Medicago sativa L. cv. Longdong[J];Acta Agrestia Sinica;2009-03
5 SA Duo-wen,MI Fu-gui*,LIU Yu-liang(College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia Autonomous Region 010019,China);Isozyme Studies of Elytrigia Hybrids(E. elongate×E. intermedia) and Their Parents[J];Acta Agrestia Sinica;2009-03
6 Cui Jinteng 1 Chen Bingxu 1 Shi Yingyao 2 Zhang Rong 3 Wang Hui 1 Qian Yiliang 2 Liu Haiyan 1 Zhu Linghua 1 Li Zhikang 1 Gao Yongming 1 1 Institute of Crop Sciences, Chinese Academy of Agricultural Science, Beijing, 100081; 2 College of Agricultural Sciences, Anhui Agricultural University, Hefei, 230036; 3 Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081;Genetic Diversity of Involved Varieties and Improvement of Elite Restorer of Indica Rice Using Backcross Introgression[J];Molecular Plant Breeding;2008-01
7 QIN Xin-min,LIANG Yan-ni,HUANG Xi-yang(College of Life Science,Guangxi Normal University,Guilin,Guangxi,541004,China);Isozymes Analysis on Different Tissues from Three Different Populations of Gekko gecko[J];Guangxi Sciences;2006-04
8 Liu Peng Sun Yugang Zhao Wenge (Harbin Normal University);COMPARATIVE STUDIES ON LDH AND EST ISOZYME PATTERNS IN FOUR TISSUES OF THREE SPECIES OF PIT VIPERS IN HEILONGJIANG PROVINCE[J];Natural Science Journal of Harbin Normal University;2007-01
9 Ji Peng1,Wang Hongyi1,Chen Kenong 2,Sheng Yunyan2(1.College of Plant science and Technology,Heilongjiang August First Land Reclamation University,Daqing 163319;2.Horticulture Department,Northeast Agriculture University);Performance of Progenies Derived from Crosses Using the Different Nelon Cultivar as Parent[J];Journal of Heilongjiang August First Land Reclamation University;2008-02
10 JI Sheng-dong1,XUE Hua-zheng2,FAN Hong-jun1,CHEN Peng1,WANG Jia-chuan1, YUAN Zhao1,YUE Chun-hui1,WANG Zhi-feng1(1.College of Life Science,Henan Normal University,Xinxiang 453007,China;2.Henan Academy of Agricultural Sciences,Zhengzhou 450002,China);Analysis of Peroxidase Isozymes between Filial Generation and Parents of Maize by Renaturation Electrophoresis[J];Journal of Henan Agricultural Sciences;2009-06
China Proceedings of conference Full-text Database 1 Hits
1 LIU Jie, YU Zhuo, WANG Xiao-juan, LI Zao-zhe (Agronomy College, Inner Mongolia Agricultural University, Hohhot 010019, China);Isozyme Analysis of F1 Hybrids between Efymus canadensis and 4 Species of Efymus[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 SHA Wei, MENG Fan-ling, FENG Chang-jun(College of Life Science and Engineering, Qiqihaer University, Heilongjiang 161006);A Study on Isozymes of Alfalfa in Different Growth Period[J];Northern Horticulture;2007-01
2 Shuai Surong;Zhang Xinquan;DU Yi(College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014);Study on Genetic Characters in Diploid and Tetraploid of Dactylis glomerata[J];ACTA AGRESTIA SINICA;1997-04
3 Jia Jianhang; Liu guozhen ;Li liyun(Biotechnology Center of Hebei Agric. Univ., Baoding 071001)Liu zhenyue(Agronomy School of Hebei Agric. Univ., Baoding 071001);Variential Identification of Letinula edodes by Isoclectric Focusing electrophoresis[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1997-01
4 MA Qi-dong, BA Tu-hu, CHENG Xia(Beijing Sinoseed Turf and Forage Co., Ltd, Beijing 100029, China);Adaptability to the Beijing area of some introduced forage varieties[J];Pratacultural Science;2004-03
5 WANG Ya-ling,SHI Shang-li,JIAO Liang(Pratacultural College of Gansu Agricultural University,Lanzhou 730070,China);Study on biological characteristics of Longdong wild alfalfa[J];Pratacultural Science;2008-01
6 Li Yangchun Wang Lingying (Gansu Agricultural University, Lanzhou 730070);POD ISOZYMES ANALYSIS OF THE DIFFERENT SPECIES ECOTYPE OF POA[J];PRATACULTURAL SCIENCE;1996-05
7 $$$$ Shuai Surong Zhang Xinquan Mao Kai Du Yi (College of Animal Science and Technology, Sichuan Agricultural);TXSTNDY ON THE ISOZYME OF TRIFOLIUM REPENSL L. BETWEEN GERMAN CULTIVARS AND SEVEN LANDRACES IN WEST CHINA[J];PRATACULTURAL SCIENCE;1998-02
8 REN Jian1,MAO Kai2, WU Yan-qi2 (1.Department of Grassland Science, Yunnan Agricultural University, Kunming 650201, China; 2.Department of Grassland Science, Sichuan Agricultural University, Yaan 625014, China);Comparison of peroxidase isozymes and esterase isozymes in Sichuan's native centipedegrass[J];Acta Pratacultural Science;2001-03
9 SUN Jian-hua, WANG Yan-rong, YU Ling(College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020, China);Growth characteristics and their correlation with the yield of Medicago sativa[J];Acta Pratacultural Science;2004-04
10 DU Hong-mei~1, LI Zhi-jian~1, ZHOU Dao-wei~1, Zhang Wei~2 (1. Key Laboratory for Vegetation Ecology, Education Ministry, Changchun 130024,China; 2. Yili Normal college, Yining 835000, China);Camparative study on isozymes of multifoliolate and trifoliolate Medicago sativa cultivars[J];Acta Pratacultural Science;2005-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved