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《Acta Agrestia Sinica》 2010-01
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Characteristics of Swainsonine-degrading Bacteria YLZZ-2 and Optimization of Conditions for Degradation

WANG Yan,HU Yan-chun,YU Yong-tao,SONG Yu-min,LI Hai-li,GENG Guo-xia,WANG Jian-hua(College of Veterinary Medicine,Northwest A & F University,Yangling,Shaanxi Province 712100,China)  
YLZZ-2 is a strain of Stenotrophomonas maltophilia capable of Swainsonine(SW) degradation.In this study,four condition factors were optimized by orthogonal test including initial culture medium pH,temperature,SW substrate concentration,and speed of shaking.The results show that YLZZ-2 could degrade SW at its best efficiency under the condition of pH value 7.0,temperature 30℃,SW concentration 5%,and shaking speed 160 r·min-1.Results of resistance test show that the bacteria had ampicillin-and-kanamycin resistance.The resistance disappeared and the ability of SW degradation lost when the plasmids were eliminated from YLZZ-2,which indicates that the SW degradation function of YLZZ-2 is associated with its plasmids.
【Fund】: 国家自然科学基金(30871901)资助;; 国家自然科学基金(30571315)资助
【CateGory Index】: S859.87
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