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《Acta Agrestia Sinica》 2010-04
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The Influence of Heterogeneous Habitat on Peroxidase Isozyme of Leymus Chinensis in Zhalong Wetland

JIAO De-zhi1,DING Xue-mei2,JIN Xiong-hua1,GUO Wan-li3,HAN Da-yong4,YANG Yun-fei4 (1.College of Life Science and Technology,Qiqihar University,Qiqihar,Heilongjiang Province 161006,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin Province 130062,China;3.College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,Zhejiang Province 310029,China;4.Institute of Grassland Science,Northeast Normal University,Key Laboratory of Vegetation Ecology of Ministry of Education,Changchun,Jilin Province 130024,China)  
The influence of heterogeneous habitat on peroxidase(POD) isozymes of Leymus chinensis was investigated to reveal how the Leymus chinensis developed strategies for restoring its population under different soil habitats.Results provide a method for assessing divergent adaptation and ecological plasticity of plants.POD analysis of Leymus chinensis were carried out in three different habitats of the Zhalong Nature Reserve.Results showed that the most POD isozyme bands were obtained when Leymus chinensis grew in alkaline soil,and the fewest bands in meadow soil.Under the same habitat,the most zymogram numbers were seen in roots of Leymus chinensis,and the fewest in leaves.The isozyme bands with Rf: 0.08,0.10,0.17 were main bands that were seen in 7 samples.The isozyme bands with Rf: 0.35,0.73 were weak bands only showing in 2 samples.The Shannon-Weinner index varied greatly in leaves with heterogeneous habitats,and that had little variation in rhizomes.Therefore,the heterogeneous habitat of the Zhalong Nature Reserve caused the differential expression of POD in the Leymus chinensis.
【Fund】: 黑龙江省教育厅科学技术研究项目(11531424);; 国家高技术研究发展计划“863”项目(2008AA10Z224);; 国家自然科学基金项目(30960261)资助
【CateGory Index】: S543.9
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