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《Acta Agrestia Sinica》 2011-02
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SRAP and RAPD Analysis of Elymus nutans Griseb.Germplasm from the Qinghai-Tibetan Plateau and Xinjiang

MIAO Jia-min1,ZHANG Xin-quan1,CHEN Zhi-hua2,ZHONG Jin-cheng2,CHEN Shi-yong1,MA Xiao1,BAI Shi-qie3(1.Department of grassland science,Sichuan Agricultural University,Ya'an,Sichuan Province 625014,China;2.College of life science and technology,Southwest University for Nationalities,Chengdu,Sichuan Province 610041,China;3.Academy of Grassland Science,Chengdu,Sichuan Province 611731,China)  
The diversity and relationships among 64 E.nutans accessions were analyzed using sequence-related amplified polymorphism(SRAP) and random amplified polymorphic DNA(RAPD) molecular markers to measure the genetic variability and diversity.Results show high genetic diversity in E.nutans(PPB=85.86%,90.39%) by using both molecular markers.Cluster analysis indicates the accessions from similar origins frequently clustered into one group.The analysis of molecular variance(AMOVA) by using both molecular markers shows that the proportion of variance either within or among geographical groups is 48.23%,39.87% and 51.77%,60.13%,respectively.Additionally,analysis of genetic variation using SRAP markers is more efficient than using RAPD markers for E.nutans.Distinct genetic differentiation accession is revealed between Qinghai-Tibetan Plateau and Xinjiang.Eco-geographical conditions such as climate and mountain ranges as well as mating system may be key factors of genetic variations.These results provide management considerations for breeding programs and germplasm collections as well as for the overall conservation of E.nutans.
【Fund】: 国家重点基础研究发展计划973项目(2007CB108907);; 国家自然科学基金项目(NSFC31072077)资助
【CateGory Index】: S543.9
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