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《Acta Agrestia Sinica》 2012-03
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Responses of Antioxidant Defense System of Lespedeza davurica to Enhanced UV-B Radiation

HAO Wen-fang,DU Run-feng,WANG Long-fei(Department of Life Science,Northwest A & F University,Yangling,Shaanxi Province 712100,China)  
The objective of this study was to investigate the effects of different UV-B radiation intensity(CK,T1,T2)on antioxidant defense system and other related indicators of Lespedeza davurica(Laxm.) Schindl.Malonaldehyde(MDA),ascorbic acid(AsA) and carotenoid(Car) contents,as well as superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) and ascorbate peroxidase(APX) activities of leaves from Lespedeza davurica under different UV-B radiation intensity were investigated.Samples were collected once every three days.The UV-B treatment was continued 15 days.Result indicated that SOD and POD activities decreased,APX and POD activities increased with UV-B radiation enhanced during the whole treatment time.SOD,POD and CAT activities decreased with UV-B radiation intensity increasing.APX activity increased during the first 9-day treatment with radiation intensity increasing then decreased with radiation intensity increasing.UV-B radiation increased AsA content,decreased Car content.Both AsA and Car contents decreased with radiation intensity increasing when compared with control.O·-2and MDA increased with radiation intensity increasing.All other tested indicators increased except SOD and POD activity as well as AsA content decreased after UV-B radiation treatment.Comprehensive evaluation of subordinate function showed that UV-B radiation reduced the antioxidant capacity of Lespedeza davurica,and the antioxidant capacity decreased with UV-B radiation intensity increasing.
【Fund】: 中国科学院“西部之光”人才培养项目(2008DF02)资助
【CateGory Index】: S541.5
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