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《Acta Agrestia Sinica》 2012-03
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Genetic Diversity of Stipa bungeana Trin.Populations in Shaanxi Analyzed by ISSR Markers

YU Jing1,JING Zhao-bin2,CHENG Ji-min1,2,3(1.College of Resources and Environment,Northwest A&F University,Yangling,Shaanxi Province 712100,China; 2.College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi Province 712100,China;3.Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling,Shaanxi Province 712100,China)  
Inter-simple sequence repeat(ISSR) markers were used to determine the genetic diversity and genetic differentiation of five Stipa bungeana natural populations in Shaanxi province.Objectives of this study were to explore the molecular and ecology mechanisms of genetic diversity of Stipa bungeana.Fifteen primers screened from 96 primers amplified stable bands.Among a total of 223 amplified bands,182(81.61%) bands were polymorphic loci.Nei's gene diversity index and Shannon's information index were 0.1887 and 0.2975,respectively,which indicated high genetic diversity existing among population.Analysis of molecular variance(AMOVA) demonstrated genetic variation mainly among populations.Genetic variation rate was 63.01% among populations,but 36.99% within population.Moreover,the level of gene flow(Nm) was measured to be 0.1139 individual per generation among populations that indicated Stipa bungeana had a higher genetic differentiation.
【Fund】: 国家现代农业产业技术体系建设-咸阳牧草试验站;; 中国科学院院地合作专项“宁夏南部山区生态保育型集约化养殖技术模式转化与示范”资助
【CateGory Index】: S543.9
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