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Feasibility Study on Domestic Rupture Disk Replaced Imported One in Particle Bombardment

XU Kai-jie;SHI Li-li;XI Ya-jun;ZHU Jian-chu;Northwest A&F University;Handan Agriculture Academy of Sciences;  
Comparing domestic rupture disk with imported one in the particle bombardment of switchgrass calluses,the burst pressure of rupture disk,the rate of hyg-resistance callus,the number of regeneration plants and the rate of transgenic plants were analyzed.The results showed that the stability of domestic rupture disk was poorer than that of imported one,whereas the average pressures of both disks were not significant difference.All tested indexes except the rate of resistant callus were also no significant difference between domestic rupture disks and imported ones.In conclusion,the domestic rupture disk could completely replace imported and these results could provide a theoretical premise to save scientific research funds in the particle bombardment.
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