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《Acta Agrestia Sinica》 2015-01
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Cultivars(Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers

LEI Yun-ting;ZHAO Yan-yan;YU Feng;LI Yuan;DOU Quan-wen;Northwest Plateau Institute of Biology,Chinese Academy of Sciences;Graduate University of Chinese Academy of Sciences;  
Molecular fingerprinting maps of 3 Elymus sihiricus cultivars and 7 germplasms were carried out using 6 simple sequences repeat(SSR) markers developed from the genomic DNA of E.sihiricus.A total of 21 polymorphic SSR fragments were detected in all the tested materials,and 2 to 5 alleles were detected by each pair of SSR primer with an average of 3.5.All the 3 cultivars and 7 germplasms could be well distinguished by the fingerprinting maps constructed by 3 pairs of core primers.In addition,seed purity assessment was conducted in cultivars 'Tongde' and 'Duoye' with 3 pairs of SSR primers.It showed that seed purity and the polymorphism of SSR alleles could be effectively identified.Moreover,it revealed that'Tongde',a domesticated cultivar,had much lower purity and higher polymorphic alleles than 'Duoye',a breeding cultivar.The SSR markers used in this study can be a powerful tool for cultivars or germplasms identification,seeds purity assessment in E.sihiricus.
【Fund】: 青海省科技厅科技促进新农村建设计划项目(2013-N-515);; 中科院“西部之光”联合学者人才培养计划项目资助
【CateGory Index】: S543
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