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《Acta Agrestia Sinica》 2015-05
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The Effect of NH_4~+-N on Anatomical Structure of Alfalfa Conducting Tissue

HAO Feng;LIU Xiao-jing;ZHANG Xiao-ling;College of Grassland Science.Gansu Agricultural University,Key Laboratory of Grassland Ecosystem of Ministry of Education,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability;  
Gannong NO.3 was used as experimental materials with five different NH_4~+-N concentrations(0,105,210,315,420 mg · L~(-1)).Anatomical structure characteristics of three types of organs(roots,stems and leaflets) were observed by using paraffin section techniques and photomicrography.The results showed that vascular tissue throughout the roots,stems and leaflets was increased at the begining,and then reduced with the further increase of nitrogen treatment.When NH_4~+-N treatment was 210 mg·L~(-1),the function of conducting tissue achieved the best performance with more vessels,more multi-vascular bundles,larger and better developed xylem and phloem area as well as more xylem conduits.Changes in other tissues of vegetative organs were also observed,the roots cortex was thicked and parenchyma cell volume decreasesd.The intramedullary cavity had flaky tissue and the area was increased.Leaves midrib became more prominent and epidermis consisted of a layer of oblong,more compactly arranged epidermal cells with increased NH_4~+-N concentration within a range of 0 to 210 mg · L~(-1).The optimal concentration was at 210 mg · L~(-1).Further increase of the concentration of NH_4~+-N concentration reduced the improvement effects instead.
【Fund】: 国家自然科学基金项目(31460622);; 公益性行业(农业)科研专项(201403048-8)资助
【CateGory Index】: S541.9
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