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Evaluation of the Resistance of Alfalfa Germplasm to Colletotrichum linicola

MA Jia-qiang;YUAN Qing-hua;WANG Yu;MIAO Li-hong;Institute of Animal Science,Chinese Academy of Agricultural Sciences;Department of Urban Landscaping Management,Forestry and Landscaping Bureau of Guangyuan City;  
Anthracnose disease resistance of fifty alfalfa materials was tested at seedling stage in greenhouse.After spray inoculation,both direct evaluation(the index of disease)and indirect evaluation(plant height,biomass,relative plasma membrane permeability,proline content,soluble sugar content and lignin content)were adopted to evaluate Anthracnose disease resistance.The object of this study was to explore the evaluation method of disease resistance in greenhouse,which aimed to provide reference for alfalfa disease prevention and disease resistance breeding.The results of direct evaluation showed that nine alfalfa cultivars-Rangelandor,Drylander,Hulunbeier,Tulufan,Honeoye,Algonquin,Longmu 801,Dupuits and Seguel-were with high resistance to Anthracnose disease.However,six cultivars-Xinjiang Daye,France(M47),Yuci,Nant,Cangzhou and Pramar were with high susceptibility.The results of indirect evaluation showed that seven alfalfa cultivars-Rangelandor,Honeoye,Algonquin,Longmu 801,Aohan,Tulufan and Seguel-were with high resistance.However,ten cultivars – France(M 47),Rambler,Wugong,Caoyuan No.3,Longdong,Xinjiang Daye,Longzhong,Pramar,Cangzhou and Yuci-were with high susceptibility.In combination with direct and indirect evaluation results,six alfalfa cultivars-Rangelandor,Honeoye,Algonquin,Longmu 801,Tulufan and Seguel-were confirmed to be with high resistance to alfalfa Anthracnose disease.And five cultivars-Yuci,Cangzhou,Pramar,Xinjiang Daye and France(M47)-were confirmed to be with high susceptibility.
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