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《Journal of Chengdu Medical College》 2007-02
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A Study on Extraction and Determination of Capsaicinoid

SHU Dan1,ZHENG Ren-yuan,YE Xiao-jin3,LUO Qin3,ZHANG Tao2(Chengdu Medical College,1.Grade 2003 in department of Clinical Medicine,2.Department of Bio-chemistry;3.Grade 2006 in department of Biotechnology,Chengdu 610083,China)  
Objective To extract and determinate capsaicinoid from capsicum with chitosan resin.Method Capsaicinoid was separated by means of 1% NaOH extracting initially.After degreasing,the capsaicinoid in extraction was absorped by chitosan resin and then eluted by ethanol.A straw yellow crystal of capsaicinoid was isolated from recrystallization.The crystal capsaicinoid was determinated by vanitlin-sodium nitrite colorimetry.Results Capsaicinoid was extracted and purified.The yield was 0.38% and the purity of crystal was 91.20%.Conclusion The method was not only simple in process,but also high in yield and purity at low cost.It could be used for industrial production.
【Fund】: 四川省教育厅资助科研项目(项目编号2006B112)
【CateGory Index】: TQ914.1
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