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《Journal of Chengdu Medical College》 2011-01
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Research on selecting the best extraction methods of chlorogenic acid in stems and leaves of L.confusa DC

CHEN Wen(Medical education research department,Huizhou Medical School,Huizhou,Guangdong 516002,China)  
Objective To select the best extraction methods and processing conditions of chlorogenic acid in stems and leaves of L.confusa DC.Methods In order to obtain the highest extraction ratio of L.confusa DC,orthogonal design and uniform design were used to optimize and select the best processing conditions among 6 different extraction methods.Results The extraction ratio of chlorogenic acid was 4.81%,4.35%,4.32%,3.93%,3.87%,3.70% for enzyme-ultrasonic wave method,enzyme method,ultrasonic wave method,ethanol refluxing method,microwave method and modified water extraction method successively.Conclusion The best method is enzyme-ultrasonic wave.The best processing condition is material ratio of 1:20,extraction time of 50 minutes for enzyme(pH=4,adding 2.0% enzyme,55℃),and 30 minutes for ultrasonic wave(250W,35kHz).The method meets the trend of green environmental conservation as well as taking advantage of medical plant resources with high efficiency.
【Fund】: 广东省中医药管理局资助项目(编号1040209)
【CateGory Index】: TQ461
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