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《Journal of Chengdu Unversity of Tarditional Chinese Medicine》 2001-01
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Experimental Study on Changes of Enteroendocrine Cells of Somatostain, Motilin and Cholecystokinin in Splenic Asthenia Rat Model

REN Ping, LIU Fang, HUANG Xi, et al (Xijing Hospital, Fourth Military Medical University,Xi'an, 710032, China)  
Objective:To investigate the relationship of "dysfunction of the spleen in transport" with gastrointestinal enterocrine cells. Methods: The short-term and long-term rat models of spleen-asthenia syndrome(RMSA I and RMSA II group) were treated by reserpine for seven days and fourteen days respectively. Si-jun-zi Decoction was used as a remedy to verify the avilability of the model. Immunohistochemical SABC method was employed to stain D cell of gastric antrum, I cell and Mo cell of duodenum. The variation of appearance and quantity of endocrine cells were observed by light microscope. Results: In the rat models of seven days, as compared with controls, the number of D cell increased (P0.01),the number of Mo cell reduced (P0.01),the percentage of strong positive D cell reduced (P0.01),the percentage of strong positive I cell and Mo cell increased (P0.01,P0.05). In the rat models of fourteen days, as compared with controls, the number of the number of Ⅰcell increased (P0.01), the percentage of strong positive D cell reduced (P0.01),the percentage of strong positive D cell and Ⅰcell increased (P0.01),the percentage of strong positive Mo cell reduced (P0.01). Conclusion: The synthetic and secretory function of the enteroendocrinr cells varyed with the development of spleen-asthenia syndrome.
【Fund】: 国家自然科学基金!资助课题 (No :39770 886 396 70 86 5 )
【CateGory Index】: R228
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