Experimental Study on Effects of Total Saponins of Panax Notoginseny on Activation of NF-kB and Infiltration of PMN in Myocardial Ischemic-reperfusion Injury
TANG Xu dong, JIANG Jian qing, LIN Chang wen, et al Chengdu Army General Hospital (Chengdu,610083,China)
Objective:To study the Influence of total saponins of Panax Notoginseny (PNS)on Activation of nuclear factor-kB (NF-kB) and infiltration of polymorphonuclear neutriphils (PMN) in myocardial ischemic-reperfusion. Methods: one hundred and twenty four Newzeland rabbits were divided into three groups: IR group (ischemia reperfusion group): Rabbits were subjected to total occlusion 45 min of left anterior desending artery (LAD) followed reperfusion, PNS proconditioning group (IR+PNS group): PNS 100mg/kg pretreated rabbits 10 min before ischemia, and sham-group. The blood and ischemic myocardial samples were studied at before ischemia ,30,60,90,120,240,360 mins after ischemia reperfusion. The expression levels of ICAM-1 were evaluated by flow cytometry, activation of NF-kB by electrophoretic mobility shift assay (EMSA),the infiltration rate of PMN were measured by MPO . Results: In IR group, after IR 30 min, activation of NF-kB were increased significantly and reached a peak at 120 min, then decreased; the expression levels of ICAM-1 were increased at 120 min and had positive correlation to the infiltration rate of PMN. In IR+PNS, PNS could inhibit the activation of NF-kB, expression of ICAM-1 and infiltration of PMN. Conclusion: The inducible activation of NF-kB starts up ICAM-1 expression of PMN and it is an important factor in myocardial ischemic-reperfusion injury, PNS has protective effect on myocardium by inhibtion activation of NF-kB, ICAM-1 expression and infiltration of PMN.