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《Journal of Chengdu University of Traditional Chinese Medicine》 2009-02
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Determination of Icariin and Paeonol in Nan Bao Capsules by RP-HPLC

WU Xiu-qing1,LI Yuan-ke1,LIANG Wei2,et al(1.Sichuan Ziyang Institute for Food and Drugs Control,Ziyang 641300;2.Chengdu University of Traditional Chinese Medicine,Chengdu 610075)  
Objective:To develop a HPLC method for the determination and analysis of the icariin and paeonol within Nan Bao capsules.Method:6 samples of Nan Bao capsules from different producers were collected.The content of icariin and paeonol were all determined by RP-HPLC method.The analysis was performed on a HPLC apparatus equipped with a C8 column(DiamonsilTM,250 mm 4.6 mm,5 μm) at 25℃.The DAD detection was carried out at a wavelength of 270 nm,the mobile phase consisted of Water(phase A),Acetonitrile(phase B) with gradient elution with the flow rate of 1.0 mL/min.Results:This method possessed desirable accuracy and reproducibility as well as simplicity.The extraction method and the conditions for HPLC were quite simple to perform.It was effective in distinguishing and determing icariin and paeonol in Nan Bao capsules.Conclusion:The method proved to be effective,accurate,and could be applied to quantitatively analyse icariin and paeonol in Nan Bao capsules.
【CateGory Index】: R286
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