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《Journal of Chongqing University of Technology(Natural Science)》 2010-05
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Study of The Disulfide Pairs and The Bioactivities of Recombinant Keratinocyte Growth Factor Type Ⅰ N-terminal deleted Mutant

ZHANG Zeng-tao,CHEN Qing,LUO Lan,CAI Yu,FAN Kai(School of Pharmaceutical and Biological Engineering,Chongqing University of Technology,Chongqing 400050,China)  
This paper studies the disulfide pair and the bioactivity of recombinant keratinocyte growth factor Type Ⅰ N-terminal deleted mutant(△23KGF-Ⅰ) expressed in E.coli.The results showed that three cysteins(Cys40,Cys102,and Cys106) of △23KGF-Ⅰ solubly expressed in E.coli were not disulfided.After 100μM Cu2+ treatment,the disulfide bond between Cys102 and Cys106 was formed.The in and out body bioactivity of △23KGF-Ⅰ containing Cys102-Cys106 disulfide bond was respectively 40% and 70% higher than its form without disulfide bond in vitro.
【Fund】: 国家“重大新药创制”科技重大专项资助项目(2009zx09102-25)
【CateGory Index】: Q78
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