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An Ofloxacin Sensing Membrane Based on Fluorescence Quenching of Fluorescent Reagent

Chen Li; Ma Ximin; Chen Jian (Department of Phamacy of Xinjiang Medical College, Urumqi 830054)  
A new ofloxacin sernsing membrane based on fluorescence quenching is reported.The sensitive optrode was combined with a membrane onto which was immobilized fluoroprobe- (cyclohexylamino, cyclohexylimide)-methyl 1-pyrenebutyrate. It can montor the concentration of ofloxacin. The recovery of the method was 96. 0 % ~ 106. 7 %. The relative standard deviations for within-day and between-day were 1. 0%~ 2. 1 %. The calibration curve was lincar in the range from 2.0μg/ml with γ=0.999 5. The detection limit of this method was 0. 046 μg/ml.
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