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《Journal of Transcluction Technology》 2005-04
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Reagentless Hydrogen Peroxide Biosensor Based on the Coimmobilization of Safranine T and Horseradish Peroxidase by Their Cross-Linking Effect

GAN Ning~(1,2),GE Cong-xin~11.Chemistry Department of Faculty of Science,Ningbo University,Ningbo Zhijiang 315211,China;2.Biochemistry Department of Faculty of Life Science,Nanjing University,Nanjing 210008,China  
A novel reagentless H_2O_2 sensor,based on the coimmobilization of horseradish peroxide(HRP) and the redox dye safranine T(ST) by their cross-linking with glutaradehyde on the surface of a glassy carbon electrode,was fabricated.The resulting modified electrode exhibited good electrochemical activity in aqueous solution,and the electron transfer rate constant between the derivative safranine T and glassy carbon electrode was about 0.066s~(1)(calculated from laviron's method).The apparent surface coverage of ST was about 9.22×10~(-11) mol/cm~2.It was found ST could mediate the electron transfer of HRP with the electrode substrate.Performance and characteristics of the biosensor were evaluated with respect to response time,detection limit,applied potential,pH and temperature.The results indicated that this biosensor is of high sensitivity to hydrogen peroxide with a low detection limit down to 1.0×10~(-7) mol/L.Moreover,dopamine,ascorbic acid and uric acid did not interfere with the determination of H_2O_2.Meanwhile,this enzyme electrode also showed good stability and reproducibility for long-term use.
【Fund】: 国家自然科学基金资助(20075023);; 配位化学国家重点实验室开放基金资助
【CateGory Index】: TP212.2
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