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《Chinese Journal of Sensors and Actuators》 2006-05
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Study of glucose and Uric Acid Biosensors Based on Screen-Printed Carbon Paste Electrodes Modified by Carbon Nanotubes

WANG You~1,XU Hui~2,LI Guang~ 2* 1.Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China; 2.Institute of Advanced Process Control, Zhejiang University, Hangzhou 310027, P.R. China  
The biosensors are prepared by immobilizing glucose oxidase and uricase respectively on the surface of screen-printed carbon paste electrodes modified by carbon nanotubes, while potassium ferricyanide is employed as electron transfer mediator. The glucose biosensors' response time is only 5 s while the response current is 1.2-30 μA for measurable linear range of 1-33.3 mM. The response time and current of the uric acid biosensors are 50 s and 0.7-14 μA for 2-20 mg/dL, respectively. The comparison experimental results show that the carbon nanotube modification speeds up the electrode reaction and improves the sensitivities of the glucose and uric acid sensors. The sensitivity of the glucose sensors is increased from 0.333 8 to 0.843 2 μA/mM by the carbon nanotube modification, while the sensitivity of the uric acid sensors is increased from 0.402 8 to 0.713 8 μA/(mg/dL).
【Fund】: 国家自然科学基金资助(60421002)
【CateGory Index】: TP212.3
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