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《Chinese Journal of Sensors and Actuators》 2007-08
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Amperometric Immunosensor for Rapid Detection of Vibrio Parahaemolyticus Based on Agarose and Nano-Au

ZHAO Guang-ying,XING Feng-fengCollege of Food Science,Biotechnology and Environmental Engineering,Zhejiang Gongshang University,Food Safety Key Lab of Zhejiang Province,Hangzhou 310035  
A disposable electrochemical immunosensor for detection of Vibrio parahaemolyticus(VP) based on the screen-printed electrode(SPE) coated with agarose/Nano-Au membrane and HRP-anti-VP has been developed.The immunosensor was characterized by cyclic voltammetry(CV).VP could be quantificationally detected according to the shift of reduction current while CV was used as electrochemical means to detect the products of the enzymatic reaction.Under the optimum experimental conditions,VP was rapidly detectable by sigmoidal curve method and form a linear calibration between 105 and 109 cfu/mL with an associated detection limit of 7.4×104 cfu/mL(S/N=3).The immunosensor had acceptable specificity,reproducibility,stability and accuracy,which indicating the immunosensor could satisfy the need of practical sample detection.
【Fund】: 国家自然科学基金资助(30571623);; 浙江省食品安全重点实验室开放项目资助(Z04-179);; 浙江省重中之重开放项目资助(Z05-178)
【CateGory Index】: TP212.9
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