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《Chinese Journal of Sensors and Actuators》 2008-05
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Rapid Detection of Escherichia coli O157:H7 Using Electrochemical Impedance Immunosensor

LI Du-juan,WANG Jian-ping*,GAI Ling,YING Yi-bin(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310029,China)  
A biosensor based on electrochemical impedance spectroscopy(EIS) was developed for rapid detection of Escherichia coli O157∶H7.It was based on the immobilization of antibodies onto a monolayer of protein A on an Quartz Crystal Au electrode surface.In the structure of the biosensor,a three electrode system was utilized-quartz crystal Au working electrode,Ag/AgCl/Cl-sat.reference electrode and Pt counter electrode.The results indicate that the immobilization of antibodies and the binding of E.coli cells to the Au electrode surface increased the imedance,which was directly measured with electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3-/4-as a redox probe.The Δimpedance,Zcells subtract Zantibody,is correlated with the concentration of E.coli cells.The detection limit of the immunosensor can reach 103 cfu/mL and the detection time is less than ten minutes.
【Fund】: 美国农业部国际合作项目资助(USDA/FAS/ICD/RSED/SCRP)
【CateGory Index】: TP212.3
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