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《Fisheries Science》 2004-01
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Effects of Nutrients on Growth and Protein and Saccharide Contents in Chlorella vulgaris

YANG Hai-bo,YU Yuan,LIU Yan,ZHANG Xin-hua,LI Ying-min,and LU Fu-rong (Department of Chemistry and Chemical Engineering,Dalian University,Dalian,116622 China)  
In the first experiment, the accumulation of protein and saccharide as growth of Chlorella . vulgaris was determined at 24~26℃ and room light; in the second experiment,effects of different concentrations of nutrients on the growth and protein and saccharide contents is C. vulgaris vere investigated under the same conditions by means of orthogonal design test. The results showed that the synthesis and accumulation of protein and saccharide occurred in the stationary phase of C. vulgaris. The maximal growth (the highest cell density of 6.46 ×107 cells/L)and accumulation(655.4 mg protein/g dry weight,and 168.1 mg saccharide/g dry weight)of the protein and saccharides in C.vulgaris were obtained at the following nutrient combinations: C6H12O6:2 g/L, KNO3:0.8 g/L,NaH2PO4:0.2 g/L;C6H12O6:10 g/L,KNO3:1.6 g/L,NaH2PO4:0.2 g/L;C6H12O6:6 g/L, KNO3:1.6 g/L,NaH2PO4:0.1 g/L.
【Fund】: 辽宁省博士科研启动基金项目(NO:001059)
【CateGory Index】: S963
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