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《Fisheries Science》 2004-11
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RAPD Analysis of Four Grouper Species

ZHENG Lian,LIU Chu-wu (The college of Fisheries, Zhanjiang Ocean University, Zhanjiang, 524025,China)  
Genotypes of four wild grouper species (Epinephelus merra Bloch, E. fario, E. malabaricus and E. fasciatus) in Zhanjiang littoral are analysed using 9 random primers by the method of random amplified polymorphic DNA (RAPD). The results sugguest that: (1) The inter- species genetic distance(Dij) are 0.4009(E .merra and E. fario), 0.4805 (E. merra and E. malabaricus), 0.5703 (E. merra and E. fasciatus), 0.4409 (E. fario and E. malabaricus), 0.4561(E. fario and E. fasciatus) and 0.4729 (E. malabaricus and E. fasciatus). (2) According to UPGMA, first, E. merra and E. fario are clustered together (0.4009); second, they and E. malabaricus are clustered (0.4611), finaly, all of them are clustered (0.4951). Therefore, the relationship of E. merra and E. fario is closer.
【Fund】: 广东省重大科技兴海资助项目(A200099A01)
【CateGory Index】: Q953
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