In Vitro Effects of Methionine-enkephalin on Phagocytosis and Encapsulation of Haemocytes in Chlamys farreri
CHEN Zhen-ying, WANG Yi-yan, SUN Hu-shan (College of Life Science, Yantai Normal University, Yantai 264025,China)
The in vitro effects of methionine-enkephalin on phagocytosis and encapsulation of haemocytes were studied in scallop (Chlamys farreri) by fluorescence microscope. The results indicated that there were the maximal rates of phagocytosis and encapsulation at an enkephalin concentration of 5μg/ml, next to at 25μg/ml and followed by 50μg/ml, significantly higher than control groups. Our results demonstrate that enkephalin can inhibit the phagocytosis and encapsulation of haemocytes in the scallop at a concentration of 5～50μg/ml, indicating that enkephalin may play a role in immune regulation.