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《Fisheries Science》 2007-01
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Phagocytosis and Microstructure of Haemocytes of Tegillarca granosa Linnaeus

PAN Sha-fang,LI Tai-wu,SU Xiu-rong(College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China)  
Cell microstructure at different starvation states and phagocytosis of haemocytes of Tegillarca granosa Linnaeus were studied by microscopic examination.According to microscopic observation with the Giemsa stain,the configuration of haemocytes became non-regulation,and granules in cytoplasm and smooth degree of cell membrane in rim were reduced gradually after starvation treatment.The haemocytes of Tegillarca granosa had strong phagocytic activities on Escherichia coli,Moraxella osloensis and Actinobacillus ureae.The configuration and components of haemocytes changed in different stages.In the first stage,as bacteria being digested,haemocytes became smaller,the small granules in cytoplasm reduced gradually,and granules of part haemocytes had exosmosis phenomenon.In phagocytic anaphase,big granules in cytoplasm assembled arround nucleolus.The antibacterial activities in the haemocytes of Tegillarca granosa were detected.The results showed that haemocytes had strong antibacterial activities on E.coli,Moraxella osloensis and Actinobacillus ureae.Their average antibacterial activities were E.col(U:0.2881)Moraxella osloensis(U:0.2157)Actinobacillus ureae(U:0.1143).
【Fund】: 国家自然科学基金资助项目(30371095)
【CateGory Index】: S917.4
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