Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Fisheries Science》 2007-09
Add to Favorite Get Latest Update

Isolation,Purification and Identification of Polysaccharides from Abalone Viscera

LI Dong-mei,TAN Feng-zhi,YANG Jing-feng,YIN Hong-ling,LI Tao,ZHU Bei-wei(College of Biology and Food Technology,Dalian Polytechnic University,Dalian 116034,China)  
Polysaccharides were extracted from abalone(Haliotis discus hannai Ino) viscera by alkaline proteinase,then deproteinized by pepsin and Sevag method.Sephadex G-100 gel chromatography and DEAE — cellulose chromatography were used to isolate and purify the polysaccharide in abalone viscera.The AHP-2 as the final product was a single homogeneous polysaccharides with molecular weight of 10 000~15 000.No protein absorption was observed in UV spectrum,but typical absorption sulphuric acid of polysaccharide was shown in IR spectrum.Chemical composition analysis indicated that the AHP-2 was Sulphated polysaccharide,and contained 11.55% of sulphate.Rhamnose,fucose,xylose,galactose and glucose were found in the AHP-2 monosaccharide,with the ratio of Rha∶Fuc∶Xyl∶Gal∶Glu=6.7∶2.0∶3.9∶7.4∶1.0 in molecule number.
【Fund】: 国际合作项目(No.2006DFA32580);; 辽宁省教育厅科技攻关项目(No.05L069)
【CateGory Index】: S917.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved