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《Fisheries Science》 2008-04
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Preliminary Study on Immune Function of the Haemolymph in Crab Portunus trituberculatus

LI Chang-hong,JIN Shan(College of Life Science and Biotechnology,Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China)  
The internal defence system of crustacean is mostly represented by the haemolymph.Assays of activities of 19 enzymes in haemocyte/serum of crab Portunus trituberculatus were performed by kit,18 and 16 enzymes were detected in female serum and cells,respectively,and all enzymes were detected in male serum and cells.Furthermore,the activities of six enzymes(Alkaline phosphatase AKP,Acid phosphatase ACP,Lysozyme LZM,Peroxidase POD,Phenoloxidase PO and SOD) in serum were determined.The results showed that the levels of AKP and ACP were extremely low.Based on the above,the antibacterial activities in the haemolymph of the crab against Vibrio alginolyticus were measured.The haemolymph of the crab was divided into serum and haemocytes by centrifugation.It was found that the serum sample showed strong antibacterial acitivities against V.alginolyticus,whereas the function time in vitro was short;the antibacterial activities of the Haemolymph lysate supernatanet(HLS) sample against V.alginolyticus was quite low.The antibacterial activities in the serum sample are higher than that in the HLS sample.
【Fund】: 浙江省自然科学基金资助项目(302440);; 浙江省科技厅资助项目(2003C32032)
【CateGory Index】: S945
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