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《Fisheries Science》 2008-12
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Purification and Determination of Molecular Weight in Serum Immunoglobulin from Tilapia

XU Zeng-hui1,CHEN Han-zhong1,LI Xiao-xu1,LI Chao1,GAN Xi2,LIANG Wan-wen2,CHEN Ming2,LI Li-ping2(1.College of Animal Science and Technology,Guangxi University,Nanning 530005,China;2.Guangxi Fishery Research Institute,Nanning 530021,China)  
Serum immunoglobulin(Ig)of healthy tilapia Oreochromis niloticus was purified by saturation ammonium sulfate precipitation and followed by Sephadex G-200 gel column chromatography.Molecular weight and partial characterization of heavy chain and light chain of Ig were measured by SDS polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting.The results showed that the heavy chain had a molecular weight of 85 kD,and the light chain 30 kD.If the serum immunoglobulin(Ig)of tilapia is tetramer in nature,the immunoglobulin would have a molecular weight of 920 kD.
【Fund】: 广西科学研究与技术开发计划项目(0639036);; 广西研究生教育创新计划项目(2007105930906M90)
【CateGory Index】: S917.4
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