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《Fisheries Science》 2009-11
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Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascites

SUN Yi-min1,2,LI Nan1,2,SONG Jie2,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 1.Hebei Chemical Engineering and Pharmaceutical College,Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Fisheries Bureau,Shijiazhuang 050011,China  
The pathogenic bacteria were isolated from diseased Japanese flounder Paralichthys olivaceus,and identified as Aeromonas hydrophila by biochemical methods and polymerase chain reaction based on the 16s rDNA sequence on GenBank via the homologies of nucleic acids of 99.8% between the isolated strain and the standard strains.The mice and the flounder were immunized with the inactivated vaccine made by this isolated Aeromonas hydrophila via dipping immersion and showed protection rate of 90% and 80% with the incidence of 6%.The antibody level was detected by agglutination reaction and indirect enzyme-linked immunosorbent assay(ELISA) with the antiserum seemed immunogenic crucially.The anatomized results showed that the immunized mice were healthy and the unimmunized mice(as the control) were diseased in liver,indicating that the vaccine was effective.
【Fund】: 河北省重点科技攻关项目(No:06780503)
【CateGory Index】: S943;S948
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【References】
Chinese Journal Full-text Database 2 Hits
1 HUANG Jun1, WEN Hua-cheng 1, SHI Jin-gu1, HUANG Yan-hua1, LIANG Zheng-sheng 2, LONG Yi-nan1, QIN Ji-xuan1 (1College of Animal Science and Technology, Guangxi University, Nanning 530005, China; 2 Administration of Fishery in Yongfu County, Yongfu, Guangxi 541800, China);Isolation and identification of pathogenic bacteria from Pelteobagrus fulvidraco ulcerative syndrome and its drug sensitive test[J];Journal of Southern Agriculture;2012-01
2 TAO Jia-fa,LAI Ying-tiao,REN Yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(PearlRiver Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory ofAquatic Animal Immune Techniques,Guangzhou 510380,China);Preliminary Study on Condition for Inactivation of Vibrio by Formalin[J];Chinese Journal of Biologicals;2011-01
【Citations】
Chinese Journal Full-text Database 3 Hits
1 GAO Dong-mei 1,2 LI Jian 1* WANG Qun 1 ( 1Yellow Sea Fisheries Research Institute,Qingdao 266071) ( 2Ocean University of China,Qingdao 26603);Study on immune efficacy induced by killed Vibrio anguillarum vaccine on Flounder (Paralichthys olivaceus)[J];Marine Fisheries Research;2004-01
2 YU De-guang 1,2 ZHU Hong-you1 WANG Guang-jun 1* XIE Jun1 YU Fang-zhao 2,3 HUANG Zhi-bin1(1 Pearl River Fisheries Research Institute,CAFS,Guangzhou 510380)(2 Fisheries College of Guangdong Ocean University,Zhanjiang 524025)(3 Extend Aquaculture(seawater)Technologies Station,Zhuhai,519050);Effects of feeding Pediococcus acidilactici on humorol immune factors of Litopenaeus vannamei[J];Marine Fisheries Research;2006-05
3 ZHU Zhi-xiu1, ZENG Zhi-ming2, HE Hou-jun1DENG Shun-zhou1, WU Xiang-dong1, WANG Ping1, LI Hui1(1.College of Animal Science and Technology, JAU, Nanchang 330045, China; 2.The Agriculture Department of Jiangxi Province, Nanchang 330046, China);Preparation of Inactivated Whole Cell Bacterin from Aeromonas hydrophila Bacteria[J];Acta Agriculturae Universitis Jiangxiensis;2003-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yun-qiang1,LI Qing-le2(1.Liusha Enterprise Ltd,Nanning,Guangxi 530021,China;2.Guanxi University,Nanning 53005,China);Pathogenicity and control of Aeromonas hydrophila[J];Guangxi Agricultural Sciences;2007-05
2 LI Nan1,2,SUN Yi-min1,2,TIAN Li-ying1,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 (1.Department of Pharmaceutical Engineering,Hebei Chemical Engineering and Pharmaceutical College,Hebei Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Hebei Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Aquatic Product Office,Hebei Shijiazhuang 050011,China);The Isolation and Identification of Aeromonas hydrophila[J];Journal of Hebei Normal University(Natural Science Edition);2009-02
3 HUANG Hui,QI Zhen-xiong,YU Lu-jun,WU Qin-chao(Animal Husbandry and Fishery Research Centre,Guangdong Haid Group Co.,Ltd.,Guangzhou 511486,PRC);Status Quo of Study on Aquatic Vaccine in China[J];Hunan Agricultural Sciences;2010-21
4 ;双抗原夹心ELISA检测抗鳗弧菌抗体效价的研究[J];Science & Technology Information;2009-07
5 CHENG Shun-feng(College of Animal Science and Technology,QAU,Qingdao 266109,China);Study of Vaccination Against Vibrio alginolyticus on Cultured Flounder Paralichthys olivaceus[J];Journal of Qingdao Agricultural University(Natural Science);2009-02
6 HUANG Jian-xun1,2,AN Li-guo1,2,YUAN Jin-duo1,2,ZHANG Fu-miao1,2,YANG Gui-wen1,2(1.Shandong Provincial Key Laboratory of Animal Resistance Biology,Jinan 250014,China; 2.College of Life Sciences,Shandong Normal University,Jinan 250014,China);Molecular Cloning and Expression Analysis of NOD1 in Cyprinus Carpio[J];Journal of University of Jinan(Science and Technology);2012-03
7 PAN Yan-hua1,MA Yue1,ZHAO Dong-ling1,SUN Xiu-qin2,WANG Qi-yao1,WU Hai-zhen1,ZHANG Yuan-xing1(1.State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shang-hai 200237,China;2.The First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China);Study on Immune Response of Paralichthys olivaceus Induced by Live Attenuated V.anguillarum Vaccine[J];Food and Drug;2009-05
8 TAO Jia-fa,LAI Ying-tiao,REN Yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(PearlRiver Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory ofAquatic Animal Immune Techniques,Guangzhou 510380,China);Preliminary Study on Condition for Inactivation of Vibrio by Formalin[J];Chinese Journal of Biologicals;2011-01
9 TAO Jia-fa,LAI Ying-tiao,REN Yan,JIANG Xiao-yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory of Aquatic Animal Immune Techniques,Guangzhou 510380,China);Improvement of Production Procedure for Inactivated Vaccine against Aeromonas hydrophila J-1 Strain[J];Chinese Journal of Biologicals;2011-03
10 ZHANG Zhen,YANG Guiwen,HUANG Jianxun,PENG Shaoqing,AN Liguo(College of Life Sciences,Shandong Normal University, Shandong Provincial Key Laboratory of Animal Resistance Biology,Jinan 250014,China);Molecular Cloning and Characterization Analysis of NLR-C Gene in Cyprinus Carpio L.[J];Journal of University of Jinan(Science and Technology);2013-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Yuan-Yuan CAO Hai-Peng DENG Lu YANG Xian-Le~(Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China);Isolation of Pathogenic Bacteria Aeromonas hydrophila from Acipenser baerii and Immune Effect of Its whole Cell Vaccine[J];Chinese Journal of Zoology;2008-06
2 SU Ying-bing~(1,2),ZOU Gui-wei~2,YUAN Ke-ping~2,ZHENG Bei-bei~(1,2),AI Xiao-hui~2(1.College of Aquaculture,Huazhong Agricultural University,Wuhan 430070;2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou 434000);Isolation and Identification of the Pathogen of Fulminant Septicaemia from Ictalurus Punctatus[J];Freshwater Fisheries;2006-05
3 XU Jin,LUO Xiao-song,ZENG Ling-Bing(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000);Isolation,identification and pathogenicity of Edwardsiella ictaluri from Chinese yellow catfish(Pelteobagrus fulvidraco)[J];Freshwater Fisheries;2009-06
4 ZHANG Wei-ni1,ZHU Zhi-hua1,CHEN Zhi-wei2,MEI Jing-liang1,LIN Xuan1(1.Fujian Agiculture and Forestry University,College of Animal Science,Fuzhou 350002;2.Fuzhou Center for Disease Control and Prevention,Fuzhou 350004);Isolation and identification of the pathogen associated with skin canker of Japanese eel(Anguilla japonica)[J];Freshwater Fisheries;2010-02
5 YU Yun-ru~1,CHU Wei-hua~1,LI Ke-min~2 (1.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing,210095,China; 2.Jiedong Veterinary Station, Guangdong Province, 522000,China);Isolation and Identification of the Pathogen of Red Neck Disease and Red Abdominal Shell Disease of Trionyx sinesis[J];Progress In Veterinary Medicine;2004-02
6 HUANG Jun 1,CHEN Qin 1,CHEN Yiming 2,CAI Zide 2,NONG Yongheng 1,KE Yongen 1 (1 College of Animal Science and Technology,Guangxi Univ.,Nanning 530005,China; 2 Fishery Technic Expanding Station of Zhaoping, Zhaoping 546800,China);Study on flesh content and its nutritive value of Pseudobagrus fulvidraco (Richardson)[J];Journal of Guangxi Agricultured and Biological Science;2001-01
7 HUANG Yan-hua1,HUANG Jun1,HU Da-sheng2 ,WEN Hua-cheng1,SHI Jin-gu1(1 College of Animal Science and Technology,Guangxi University,Nanning 530005,China;2 Guangxi Fishery Technical Extension Center,Nanning 530022,China);Isolation and identification of pathogenic bacteria from Rana tigrina Cantor infected with red-leg disease and drug sensitivity tests[J];Guangxi Agricultural Sciences;2010-11
8 Yang Guang 1) Yang Xianle 2) Chen Chengfu 3) ( 1) Department of Animal, Hubei Agricultural College, Jingzhou 434103; 2) Changjiang Fishery Research Institute, Jingzhou 434100; 3) Fishery College, Huazhong Agricultural U;Studies on The Pathogen of Red Abdominal Shell Desease of Trionyx sinensis[J];JOURNAL OF HUBEI AGRICULTURAL COLLEGE;1998-02
9 DENG Xian-Yu1, 2, LUO Wen2, 3, TAN Shu-Hua1, QIU Shan-Hong4, CHEN Kang-Gui1(1.School of Life Sciences, Hunan University of Science and Technology, Xiangtan, 411201;2.School of Zoological Sciences, Zhejiang University, Hangzhou, 310058;3.School of Life Sciences, Shaoxing University, Shaoxing, 312000;4.No.3 Middle School of Huaihua City of Hunan Province, Huaihua, 418000);ISOLATION AND IDENTIFICATION OF BACTERIOSIS PATHOGEN——EDWARDSIELLA TARDA FROM YELLOW CARTFISH(PELTEOBAGRUS FULVIDRACO) WITH RED HEAD DISEASE[J];Oceanologia Et Limnologia Sinica;2008-05
10 GENG Yi, WANG Kai-Yu, FAN Fang-Ling, CHEN De-Fang, HUANG Jin-Lu (Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, 625014);ISOLATION,IDENTIFICATION AND BIOLOGICAL CHARACTERIZATION OF EDWARDSIELLA ICTALURI FROM CULTURED YELLOW CATFISH PELTEOBAGRUS FULVIDRACO[J];Oceanologia Et Limnologia Sinica;2010-01
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 HUANG Jun, SHI Jin-gu, HUANG Yan-hua, WEN Hua-cheng, CHEN Wu-shi, QIN Ji-xuan, QIN Li-fen, TENG Zhong-zuo (College of Animal Science and Technology, Guangxi University / Guangxi Laboratory of Aquatic Animal Disease Diagnosis, Nanning 530005, China);Isolation and identification of lepidorthosis pathogenic bacteria in tilapia and its drug sensitivity test[J];Journal of Southern Agriculture;2012-08
2 LIANG Zheng-sheng 1,HUANG Jun2 *,SHI Jin-gu2,HUANG Yan-hua2,PENG Min-yi2,WEN Hua-cheng 2,LIANG Xiao 2,QIN Ji-xuan 1(1 Yongfu Fishery Administration Station,Yongfu,Guangxi 541800,China;2 College of Animal Science and Technology,Guangxi University /Guangxi Laboratory of Aquatic Animal Disease Diagnosis,Nanning 530005,China);Isolation and identification of ascites disease pathogen from Pelteobagrus fulvidraco Richardson and its drug sensitivity test[J];Journal of Southern Agriculture;2012-09
3 LUO Hua-ping1,SHI Jin-gu2,PENG Min-yi2,HU Da-sheng3,HUANG Yan-hua2,HUANG Xin-cai2,4,HUANG Jun2*(1Wuming Fishery Supervision and Administration Station,Wuming,Guangxi 530100,China;2College of Animal Science and Technology/Guangxi Aquatic Animal Disease Diagnosis Lab,Guangxi University,Nanning 530005,China;3Guangxi General Station of Aquaculture Technology Extension,Nanning 530022,China;4Sanwu Husbandry and Aquaculture Technology Extension Station,Xingbin District,Laibin City,Guangxi 546109,China);Isolation and identification of Plesimonas shigelloides pathogenic bacteria from young Truogx sinensis and its drug sensitivity test[J];Journal of Southern Agriculture;2013-04
4 GOU Xiao-lan, WANG Li( Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041,China);Pathogenicity and Detection of Virulence Genes of BacteriumYersinia enterocolitica Isolated from Yellow Catfish (Pelteobagrus fulvidraco)[J];Fisheries Science;2013-05
5 GOU Xiao-lan1,WANG Li1,HUANG Yan-qing2(1.Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education,College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,P.R.China; 2.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Shanghai 200090,P.R.China);Phylogenetic Analysis and 16S rDNA of Yersinia enterocolitica Isolated from Yellow Catfish(Pelteobagrus fulvidraco)[J];Journal of Hydroecology;2012-05
6 HUANG Jun;HUANG Yan-Hua;HU Da-Sheng;LUO Hua-Ping;SHI Jin-Gu;PENG Min-Yi;XUAN Jun-Cheng;QIN Li-Fen;TENG Zhong-Zuo;ZENG Gui-Zhong;Lab of Aquatic Animal Diseases Diagnosis, College of Animal Science and Technology, Guangxi University;Guangxi Marine Products Technical Promotion Station;Wuming fishery Supervision and Management Station;;CHARACTERIZATION OF WHITE PLASTRON DISEASE PATHOGENS AND DETECTION OF SIX KNOWN VIRULENCE GENES IN TRUOGX SINENSIS[J];Acta Hydrobiologica Sinica;2013-05
7 HUANG Jun1,PENG Min-yi1,LUO Hua-ping2,HU Da-sheng3,HUANG Yan-hua1,WEN Hua-cheng1,SHI Jin-gu1,PENG Ya1(1.College of Animal Science and Technology,Guangxi University,Guangxi Nanning 5300005,China;2.Wuming County of Guangxi Fishery Management Station,Guangxi Wuming 530100,China;3.Guangxi Fishery Technical Extension Center,Guangxi Nanning 530022,China);Gaining and Disappearing Rates in Drug Resistance of Florfenicol with Aeromonas sobri by Yellow Catfish(Pelteobagrus fulvidraco)[J];Southwest China Journal of Agricultural Sciences;2013-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Lei; Li Guangyou; Mao Yuanxing(Institute of Oceanology, Academia Sinica, Qingdao 266071);STUDIES ON THE ACTIVITIES AND CHARACTERISTICS OF THE ANTIBACTERIA, BACTERIOLYSIS AND PHENOLOXIDASE IN THE HAEMOLYMPH OF PENAEUS CHINENSIS[J];Oceanologia Et Limnologia Sinica;1995-02
2 LIU Heng; LI Guang - you(Insititule of Oceanology, The Chinese Academy of deiences, Qingdao, 266071);THE EFFECT OF IMMUNOPOLYSACCHARIDE AS A FOOD ADDITIVE ON THE PENAEID SHRIMP, PENAEUS VANNAMEI[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1998-02
3 LIU Shu-qing; JIANG Xiao-lu; MOU Hai-jin; WANG Hui-mi; GUAN Hua-shi(Fishery College, Ocean University of Qingdao, Qingdao, 266003);EFFECTS OF IMMUNOPOIYSACCHARIDE ON LSZ, ALP, ACP AND POD ACTIVITIES OF PENEUSIS SERUM[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1999-03
4 MO Zhao lan XU Yong li ZHANG Pei jun(Experimental Marine Biological Laboratory, Institute of Oceanology, Chinese of Academy of Sciences., Qingdao, 266071);VACCINATION AGAINST Vibrio Anguillarum ON CULTURED FLOUNDER, Paralichthys olivaceus[J];Marine Sciences;2002-04
5 LI Jian SUN Xiu tao WANG Qun DAI Fang yu LUI De yue MA Xiang dong (Yellow Sea Fisheries Research Institute, Qingdao 266071);Studies on the effects of probiotics in Crustacean's farming[J];Marine Fisherries Reseach;2001-02
6 LIU Yi 1,CHEN Chang fu 2,ZHANG Yuan zhu 1,CAO Lan 3 (1.Institute of Biological Resources, Jiangxi Academy of Sciences,Nanchang 330029, PRC; 2.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,PRC; 3.Jiangxi College of Traditiona;Immune Research Status of Fish Bacterial Disease[J];JIANGXI SCIENCE;1999-02
7 ZHU Zhi-xiu 1, HE Hou-jun 1,DENG Shun-zhou 1 WANG Xiao-ying 1,MIN Si-fan 2, FU Guang-hua 1 (1.College of Animal Science & Techonology, JAU, Nanchang 330045, China; 2.Department of Food Science, JAU, Nanchang 330045, China);Isolation and Identification of Aeromonas hydrophila in Frog[J];Acta Agriculturae Universitis Jiangxiensis;2002-03
8 ZHU Zhi-xiu 1, ZENG Zhi-ming 2, HE Hou-jun 1,DENG Shun-zhou 1WU Xiang-dong 1, ZHANG Jin-hua 1,LIU An-pin 1 (1.College of Animal Science and Technology,JAU,Nanchang 330045,China;2.The Agriculture Department of Jiangxi Province,Nanchang 330046,China);Biological Characteristics of Current Strains of Aeromonas hydrophila from Jiangxi[J];Acta Agriculturae Universitis Jiangxiensis;2003-04
9 Chen Huaiqing and Lu Chengping(Department of Veterinary Medicine);STUDY ON THE PATHOGEN OF EPIDEMIC SEPTICEMIA OCCURRED IN CULTURED CYPRINOID FISHES IN SOUTHEASTERN CHINA[J];Journal of Nanjing Agricultural University;1991-04
10 Yang Xianle (Yangtze River Fisheries Research Institute,Jinshashi 434000)Chen Yuanxin (Honghu Spread Station of Aquatic Technology 433200);THE EXISTING SITUATION AND TENDENCY IN DEVELOPMENT OF FISH VACCINE[J];JOURNAL OF FISHERIES OF CHINA;1996-02
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