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《Fisheries Science》 2010-06
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Effects of Polysaccharides from Astragalus membranaceus on Non-specific Immune in Sea Cucumber Apostichopus japonicus

FAN Ying,WANG Shu-xian,YE Hai-bin,XU La,ZHU An-cheng,YANG Xiu-sheng,LI Tian-bao(Shandong Province Mariculture Research Institute,Shandong Province Key Laboratory for Disease Control in Mariculture,Qingdao 266002,China)  
The activities of lysozyme(LSZ),superoxide dismutase(SOD),and alkaline phosphatase(AKP)and content of complement C3 were monitored in the coelomic fluid of sea cucumber Apostichopus japonicus 1,3,and 6 consecutive days after injection of sterile fluid of polysaccharides from Astragalus membranaceus at a rate of 500 μl individually to evaluate the effect of the polysaccharides on immunological system of the individual.The higher activities of LSZ,SOD,and AKP and content of C3 were observed on the third day after injection at a dose of 0.2,0.4,and 0.6 mg per individual,the maximal activities of LSZ(232.25 U/ml),SOD(45.1 U/ml),and AKP(966.5 nkat/L)at 0.6 mg level,and the maximal content of C3(0.09 g/L)at 0.4 mg level.There were significant difference(P0.05)compared with the control group by SPSS,indicating that the polysaccharides from A.membranaceus enhanced the immunological functions in the sea cucumber.
【Fund】: 山东省财政厅科技创新项目[鲁财农指(2008)109号]
【CateGory Index】: S917.4
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