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《Fisheries Science》 2011-05
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Environmental Bacterial Diversity in Sea Cucumber (Apostichopus japonicus) Culture Under Different Salinities Based on DGGE Fingerprint

GUAN Xiao-yan,ZHOU Zun-chun,JIANG Bing,DONG Ying,WANG Xiao-yu,JIANG Bei,YANG Ai-fu,CHEN Zhong,WANG Bai,SUN Da-peng(Liaoning Ocean and Fisheries Science Research Institute,Liaoning Marine Environmental Monitoring General Station,Liaoning Key Lab of Marine Fishery Molecular Biology,Dalian 116023,China)  
In this paper,the bacterial diversity in the sea cucumber culture environment at different salinity levels was investigated using a culture-independent method of 16S rDNA PCR-DGGE fingerprinting.The water samples were collected from sea cucumber culture tanks with four salinity gradients(22,26,30,and 34) in a 10 dayinterval.The PCR-DGGE fingerprint analysis showed that the bacteria were abundant in the sea cucumber culture environments,most of which belong to nonculturable bacteria.It was found that species of Proteobacteria and CFB(Cytophga Flavobacterium-Bacteroides)were predominated in all the samples.Pseudoalteromonas sp.was detected from cultural environment,which have been reported a pathogen of the Skin Ulceration Syndrom.The results also showed that the samples at the same salinity levels shared similar compositions,and displayed a cluster in the clustering finger.Diversity index of the samples varied from 1.7 to 2.3,and the change was not significant at the same salinity at different sampling time.
【Fund】: 国家海洋局海洋公益专项(200705007);; 辽宁省科技计划项目(200820300)
【CateGory Index】: S917.4
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