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《Fisheries Science》 2011-11
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Identification and Culture Condition Optimization of Metabolites in Marine Gorgonian-symbiotic Bacillus sp.

CHENG Peng-fei1,LIANG Shao-kang1,CHENG Guo-jun1, NONG Xu-hua1,2,QI Shu-hua2,XIONG Hai-rong1(1.College of Life Science,South Central University for Nationalities,Wuhan 430074,China;2.Key Laboratory of Tropical Marine Environmental Dynamics,Chinese Academy of Sciences,Guangzhou 510301,China)  
A marine gorgonian-symbiotic bacterium(SCSIO No.00856) was taxonomically identified by morphological analysis and 16S rRNA gene sequencing,and the bioactive metabolites of the bacterium were further studied.This strain was identified as Bacillus sp.,and its bioactive metabolites were found to inhibit the growth of yeast Saccharomyces cerevisias and Staphylococcus aureus when the(SCSIO No.00856) was cultured in the selected cultivation medium(pH 7.0) at 28 ℃,especially for yeast S.cerevisias on day 6.The filter paper diffusion method and orthogonal experiment on optimization of the maximal bioactive metabolites of the yeast showed that the optimum medium was comprised of sea salt at a rate of 26.25 g/L,glucose 12 g/L,yeast extract 3 g/L,monopotassium phosphate 3 g/L,and malt extract 5 g/L,and fermented for 6 days.
【Fund】: 中国科学院热带海洋环境动力学重点试验室开放课题资助项目(LMB091003)
【CateGory Index】: R284
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