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《Fisheries Science》 2012-02
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Optimization of Protective Medium for Vibrio anguillarum Vector Vaccine MVAV6203A-1 in Freeze Drying by Response Surface Methodology

WANG San-ying,LIU Qin,WANG Qi-yao,ZHANG Yuan-xing( State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China )  
The protective medium for Vibrio anguillarum vector vaccine MVAV6203A-1 was optimized by mono-factorial experiment,factor combination test and response surface methodology(RSM).The results showed that the cell resistance to freeze drying was improved by combination of galactose,trehalose and skimmed milk.The quadratic model for the three significant factors was established referring cell survival rate as the target response by CCD design and response surface analysis.The optional protective medium was optimized as the following: 4.6% of galactose,2.3% trehalose and 10.2% skimmed milk.In the lyophilization test repeated for 3 times,the mean V.anguillarum MVAV6203A-1 survival rate of 77.3% was achieved,indicating that the optional protective additive was suitable for the production of V.anguillarum vector vaccine MVAV6203A-1.
【Fund】: 国家自然科学基金资助项目(30600460);; 国家“863”计划重点项目(2006AA100310);; 现代农业产业技术体系建设专项资金资助项目(CARS-50-07)
【CateGory Index】: S948
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Hui-ping,MA Li,Dai Guang-li,YAN Qing-quan,XU Yi-wen(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);Optimization of Cryoprotectants for Lactobacillus rhamnosus TUST006 by Response Surface Methods[J];Modern Food Science and Technology;2010-09
2 LI Jia-peng,ZHANG Xue-gang,QIAO Xiao-ling,NIU Jin-yang (China Meat Reseach Center, Beijing 100068, China);Optimization of Formula of Cryoprotectants for Lactobacillus plantarum by Response Surface Methodology (RSM)[J];Food Science;2008-06
3 JI Zhen-jie, GUO De-jun (College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, China);Optimization of Formula of Protective Additive of Bifidobacterium by Response Surface Method[J];Chinese Journal of Biologicals;2008-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Pu Li-li, Liu Ning, Zhang Ying-hua, Huo Gui-cheng (College of Food Science ,NortheastAgricultural University, Harbin 150030,China);The Research Development of Cryoprotector of Lactic Acid Bacteria and its Protective Mechanism[J];Guangzhou Food Science and Technology;2005-01
2 LIU Hui-ping,MA Li,Dai Guang-li,YAN Qing-quan,XU Yi-wen(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);Optimization of Cryoprotectants for Lactobacillus rhamnosus TUST006 by Response Surface Methods[J];Modern Food Science and Technology;2010-09
3 Wang Liyan1,Guo Shuguo1,Li Chenghua2(1.Shenyang University of Chemical Technology,Shenyang,110142,China;2.The Engineering College Shenyang University Chemical Technology,Shenyang,110142,China);Optimization for Technological Parameters of Apple Freeze-drying based on Response Surface Methodology[J];Chinese Agricultural Mechanization;2012-06
4 LIANG Yan-ying et al(College of Enology,Norfhweet A & F Univasity Shaanxi Engineering Research Center for Viti-iniculture,Yangling,Shaanxi 712100);Open-using and Maintenance of Lyophilizer in the Large-scale Instrument Sharing System[J];Journal of Anhui Agricultural Sciences;2013-07
5 LI Liwen;PAN Gang;LI Liang;LI Hong;SHI Wenqing;ZHANG Honggang;ZHU Guangwei;Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences;Research Center for Eco-environmental Sciences,Chinese Academy of Sciences;;Effect and mechanism of algae bloom on the denitrification processes in the sediments of Lake Taihu[J];Journal of Lake Sciences;2013-05
6 Gao Zhenpeng,Yue Tianli,YuanYahong,Liu Yingjie(College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China);Screening of Aroma-producing Active Dried Yeast Protector for Cider[J];Academic Periodical of Farm Products Processing;2007-03
7 Man Lili,Xiang Dianjun(Heilongjiang Agricultural Economy Professional College,Mudanjiang,Heilongjiang 157041,China);Advances of Freeze-dried LAB[J];Academic Periodical of Farm Products Processing;2010-03
8 Zhou Jianlei,Qiao Fadong (Bioengineering College,He'nan University of Technology,Zhengzhou,He'nan 450052,China);Progress of Concentrated Raise in Produce Concentrated Meat Starter[J];Academic Periodical of Farm Products Processing;2010-09
9 GAO Yan1,ZHANG Ting-ting1,XIONG Xing-yao1,TAN Xing-he2,ZHOU Hong-li2,TANG Wei3 and SU Xiao-jun1(1.Hu'nan Engineering Lab for Alcohol Fuels from Biomass,Changsha,Hu'nan 410128;2.College of Food Science and Technology,Hunan Agricultural University,Changsha,Hu'nan 410128;3.Yangtze River Bio-energy Co.Ltd.,Changsha,Hu'nan 410001,China);Present Status and Prospects of the Development of Alcohol Active Dry Yeast[J];Liquor-Making Science & Technology;2010-12
10 Li Peipei,Zhang Dongdong,Wang Hui,Wang Xiaojuan,Wang Xiaofen,Cui Zongjun※(College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China);Fermentation and preservation of cellulose-degrading microbial community[J];Transactions of the Chinese Society of Agricultural Engineering;2011-S1
China Proceedings of conference Full-text Database 2 Hits
1 Liu xiaoyi (College of Food Science of SWU,Chongqing,400715);The Research Development of Cryoprotector of Lactic Acid Bacteria and its Protective Mechanism[A];Chinese Society of Agricultural Engineering[C];2009
2 Li Peipei,Zhang Dongdong,Wang Hui,Wang Xiaojuan,Wang Xiaofen,Cui Zongjun (College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China);Fermentation and preservation of a cellulose-degrading microbial community[A];Chinese Society of Agricultural Engineering[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Chao-min et al (College of Soil and Environment,Shenyang Agricultural University,Shenyang,Liaoning 110161);Development of the Sodium-aliginate Immobilized Cell Technology[J];Journal of Anhui Agricultural Sciences;2006-07
2 REN Yu-feng et al(College of Biological Science and Engineering,North University for Ethnics,Yinchuan,Ningxia 750021);Effects of Coating with Sodium Alginate on the Fresh-keeping Effects of Lingwu Long Jujube at Low Temperature[J];Journal of Anhui Agricultural Sciences;2009-15
3 SU Junfeng REN Li WANG Lixin (Institute of Polymer Science and Engineering, Hebei University of Technology, Tianjin, 300130);Technology and Recent Progress of Microencapsulation[J];Materials Review;2003-S1
4 ZHANG Ke da, XU Dong mei, WANG Ping (Institute of Chemistry and Chemical Engineering, Soochow University, Suzhou 215006, China);Microencapsulation Method[J];Journal of Functional Polymers;2001-04
5 LUO Jia-qi,YU Xiao-chen,YU Cai-yuan(Department of Chemical Engineering,Dalian University of Technology,Dalian 116012,China);Study of the Microencapsulation Technology of Lactobacillus Acidophilus[J];Drying Technology & Equipment;2008-06
6 Qi Wei, Yang Ze-yu, Du Lian-xing (Tianjin University of Science and Technology, Tianjin Key lab of Industrial Microbiology, Tianjin 300222, China);Preparation of Microencapsulation of Bifidobacterium and Study on its Stability[J];Guangzhou Food Science and Technology;2005-04
7 LIU Jia-jun(Guangdong Industry Technical College-Food and Biology Department,Guangzhou 510030,China);Preserving Effect of Sodium alginate on Fresh Mango[J];Modern Food Science and Technology;2009-06
8 LIU Hui-ping,MA Li,Dai Guang-li,YAN Qing-quan,XU Yi-wen(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);Optimization of Cryoprotectants for Lactobacillus rhamnosus TUST006 by Response Surface Methods[J];Modern Food Science and Technology;2010-09
9 Hu Jianqing,Li Hui,Tu Weiping,Wang Feng (College of Chemistry and Chemical Engineering,South China University of Technology, Guangzhou 510640,China);DCOIT microcapsules prepared by gelatin/gum arabic composite coacervation[J];China Adhesives;2012-06
10 TIAN Yun,LU Xiangyang,HE Xiaojie ,YI Ke,HUANG Chengjiang(The Lab of Biochemistry and Fermentation Engineering, Hunan Agricultural University, Changsha 410128; The Second Hospital of Xiangya Medical College, Central South University, Changsha 410011);The Production and Applications of Microcapsule[J];Science Technology and Engineer;2005-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 TIAN Hong tao 1, ZHANG Chi 2, JIA Ying min 1, ZHANG Bo lin 1 (1 Department of Food Science,Agriculture University of Hebei,Baoding 071001,China; 2 Institute of Food Science and Technology,China Agriculture University, Beijing 100094,China);The initial study on bio-protective role of trehalose during preparation and storage of freeze-dried powder of Bifidobacterium longum[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;2000-02
2 ZHANG Ying-hua1,2,HUO Gui-cheng1,2,GUO Ling1,2(1.Key Lab of Dairy Science,Ministry of Education,Harbin Heilongjiang 150030,PRC; 2.Food College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Research advance of the freeze-drying technology of lactic acid bacteria[J];Journal of Northeast Agricultural University;2005-06
3 Pu Li-li, Liu Ning, Zhang Ying-hua, Huo Gui-cheng (College of Food Science ,NortheastAgricultural University, Harbin 150030,China);The Research Development of Cryoprotector of Lactic Acid Bacteria and its Protective Mechanism[J];Guangzhou Food Science and Technology;2005-01
4 YANG Zhi-yan1,YIN Shu-hua2, BAI Ming2,GUO Li-mei2 (1.Research Center of Modern Analysis, Tianjin University of Science and Technology,Tianjin 300457, China) (2.College of Material Science and Chemical Engineering, Tianjin University of Science and Technology, Tianjin 300457, China);Optimization of Extraction of Total Flavonoids from Chinese Pine Pollen by Response Surface Design(RSD)[J];Modern Food Science and Technology;2008-03
5 Zhu Yan;Meat Starter Cultures and Its Mode of Fermentation[J];Meat Research;2003-01
6 ZHANG Xuemei,YANG Yong (College of Food Science,Sichuan Agricultural University,Ya’an 625014,China);Microbial Culture Starters in Fermented Meat Products[J];Meat Research;2009-08
7 LIU Dong-mei,LI Li,LIANG Shi-zhong,YANG Xiao-quan(College of Food and Bioengineering,South China University of Technology,Guangzhou 510640, China);Review of study on DVS yogurt starter cultures[J];China Dairy Industry;2005-01
8 LI Ai-li, DAI Min, HUO Gui-cheng (College of Food Science, Northeast Agriculture University, Harbin 150030,China);Industrial preparing of concentrated lactic acid bacteria starter[J];China Dairy Industry;2005-06
9 HUANG Li-jin,LU Zhao-xin,YUAN Yong-jun (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China);Screening of the Proper Freeze-drying Protectants for Yoghurt Starter[J];Food Science;2005-12
10 LI Jia-peng,ZHANG Xue-gang,QIAO Xiao-ling,NIU Jin-yang (China Meat Reseach Center, Beijing 100068, China);Optimization of Formula of Cryoprotectants for Lactobacillus plantarum by Response Surface Methodology (RSM)[J];Food Science;2008-06
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