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《Fisheries Science》 2012-10
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Isolation,Identification and Drug Susceptibility of a Pathogenic Bacterial Strain from Bluntnose Black Bream Megalobrama amblycephala

XIA Fei1,2,LIANG Li-guo2,XIE Jun2(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)  
A dominant bacterium WJ-8 was isolated from hepatopancreas of diseased bluntnose black bream Megalobrama amblycephala.The challenge test by WJ-8 revealed that the prevalence of symptoms and spontaneous onset symptoms were as the same as the naturally infected bluntnose black bream,proving that WJ-8 was pathogenic to the bluntnose black bream.The morphological,physiological,and biochemical characteristics,and the construction of phylogenetic tree supported that the isolated strain was referred as Aeromonas hydrophila.The drug sensitive test by paper extension methods showed that florfenicol,Mepiquat,Rifamycin,streptomycin,kanamycin and other 22 kinds of drugs had the most inhibitive effects on the strain which showed resistance to penicillin,ampicillin,carbenicillin,oxacillin,and other 12 kinds of drugs.
【Fund】: 现代农业产业技术体系建设专项(CARS-46-10)
【CateGory Index】: S943
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