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《Chinese Journal of Catalysis》 2008-10
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Properties of β-Glucosidase Immobilized on Magnetic Nanoparticles of Aluminum Nitride

PAN Lihua,LUO Jianping,WANG Guijuan,XU Xueling,WAN Wen(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,Anhui,China)  
The conditions for β-glucosidase immobilization with magnetic nanoparticles of aluminum nitride were optimized and the properties of immobilized β-glucosidase were investigated.The maximum immobilized protein recovery(82.6%)and activity recovery(78.4%)were obtained with β-glucosidase solution(5 ml,50 mg/ml)and glutaraldehyde solution(0.5 ml,2%)added to suspension of magnetic aluminum nitride nanoparticles(4.5 ml,100 mg/ml)and immobilized for 3.5 h at the temperature of 20 ℃ and pH value of 5.0.The structure of immobilized enzyme was incompact and not changed evidently.The Michaelis constant(Km)for hydrolysis of 4-nitrophenyl-β-D-glucoside by the immobilized β-glucosidase,with good storage stability and operation stability,was 0.88 mmol/L at the optimal temperature of 55 ℃ and the optimal pH value of 5.0.The results suggested that the magnetic aluminum nitride nanoparticle is a good support for the immobilization of β-glucosidase.
【Fund】: 安徽省自然科学基金(050410301);; 安徽省青年教师资助计划(2006jp1022)
【CateGory Index】: TB383.1
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