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《Chinese Journal of Catalysis》 2010-10
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Efficient Synthesis of α-D-Glucose-1-Phosphate by Maltodextrin Phosphorylase Immobilized on Amino-functionalized Magnetic Nanoparticles

DONG Qing, OUYANG Liming, LIU Jianwen, XU Jianhe State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China  
α-D-Glucose-1-phosphate (Glc-1-P) is an expensive intermediate in the biosynthesis of nucleotide glucose. This paper describes a biocatalytic system for the efficient synthesis of Glc-1-P from the low cost raw materials: maltodextrin and phosphate at ordinary tempera-tures. After molecular cloning and the expression of a maltodextrin phosphorylase (MalPase) gene from E. coli (Escherichia coli) K12, the resultant recombinant enzyme was immobilized on amino-functionalized magnetic nanoparticles for recycling and repeated use. Conditions for the biocatalytic reaction were optimized and the immobilized MalPase could be easily recovered and reused over eight cycles in the repeated synthesis of Glc-1-P. After simple purification steps approximately 440 mg of crude product was obtained with a moderate isolation yield of 70.5%.
【Fund】: Supported by the National Natural Science Foundation of China (20773038 20902023);; the National Basic Research Program of China (973 Program 2009CB724706 2009ZX09501-016);; the China National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204);; the Shanghai Leading Academic Discipline Project (B505)
【CateGory Index】: TQ265.11
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