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《Journal of Changjiang Vegetables》 2011-18
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Identification of Seed Purity of Jiamei Bitter Gourd by SRAP Marker

XU Duanxiang,GAO Shan,LIN Biying,FU Ruiqing(Fuzhou Institute of Vegetable Sciences,Fuzhou,Fujian 350111)  
Using SRAP molecular marker technology,the DNA fingerprints of Jiamei bitter gourd and its parents were analyzed.After repeated experiments,2 pairs of primers were screened out from 108 primer pairs,which could amplify the characteristic polymorphic bands of F1 generation and its parents,with the PCR products possessing high stability and repeatability,and the corresponding DNA fingerprints were built.100 individuals of Jiamei bitter gourd were identified. The results showed that only 3 individuals had female-specific band without male-specific band,which agreed with the result of the field test,and the hybrid rate was 97%.
【Fund】: 福建省科技计划重大专项专题[2008NZ0002-1(1)]
【CateGory Index】: S642.5
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 GAO Shan,LIN Bi-ying,XU Duan-xiang,FU Rui-qing,LIN Feng (Fuzhou Institute of Vegetable Research,Fuzhou 350012,Fujian,China);A New Bitter Gourd F_1 Hybrid —‘Jiayu'[J];China Vegetables;2010-18
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