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《Materials Review》 2006-12
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Study on Preparation of Magnetic Particle of Crosslinked Sodium Alginate and Immobilization of Trypsin

LIU Zheng~ 1,2 , LIN Yuanbin~1, LU Huidan~2(1 The College of Chemistry, Xiangtan University, Xiangtan 411105;2 Department of Material and Chemical Engineering, Guilin Institute of Technology, Guilin 541004)  
The aim of this paper is to prepare magnetic particle of crosslinked sodium alginate and to apply it to immobilize trypsin. The magnetic particle of crosslinked sodium alginate are yielded by method of embedding on Fe_3O_4 particle. Trypsin is immobilized on magnetic particle of crosslinked sodium alginate through crosslinked reaction with glutaraldehyde. The magnetic particle of crosslinked sodium alginate are characterized by methods of TEM, particle size analysis and IR analysis. The effects of amount of enzyme given, concentration of glutaraldehyde and pH value in the process of preparation on the activity of magnetic immobilixed enzyme are studied.The characteristics of enzymology of the magnetic immobilized trypsin are discussed on the basis of being compared with free enzyme.Research reveals magnetic particle of crosslinked sodium alginate to be a good carrier of immobilized trypsin.The optimum conditions of immobilized trypsin are amount of enzyme given for 100mg/0.1g magnetic carrier, concentration of glutaraldehyde for 5%,pH value for 6 and adsorption time for 12h.
【Fund】: 广西自然科学基金项目(编号:桂科自0342047)
【CateGory Index】: Q814
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