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《Materials Review》 2009-18
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Study on the Immobilization of Catalase by AB-8 Macroporous Absorbent Resin

XIE Xuefeng,ZHANG Zhaohui,CHEN Peice(College of Biotechnology and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014)  
Condition optimization and stability for immobilization of catalase by AB-8 macroporous absorbent resin and glutaraldehyde have been studied.The experimental results indicate that the optimal conditions of catalase immobilization are:the appropriate temperature is 35℃,pH value is 7.0,adsorbent time is 6h,quantity of catalase is 970U per gram carrier,crosslinking time is 2h and the concentration of the crosslinking agent(glutaraldehyde) is 0.2vol%.The maximum yield of immobilized enzyme is 45.2% while catalase is immobilized on those conditions.Compare with the free enzyme,the immobilized enzyme has got certain improvement on thermal stability and pH stability,the remaining activity is more than 60% of the primary enzymeactivity after the immobilized enzyme is used repeatedly for 10 times.It show that the immobilized catalase have good operational stability.
【Fund】: 浙江省科技厅重点计划项目(2006C21117)
【CateGory Index】: TB302
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Chinese Journal Full-text Database 6 Hits
1 Wang Yujie;Lu Chibiao;Bai Guangyue;Wu Zisheng;Zhang Hezhe(Department of Chemistry,Northeast Normal University,Changchun, 130024);Catalase Immobilized with Microcapsule[J];JOURNAL OF NORTHEAST NORMAL UNIVERSITY (NATURAL SCIENCE EDITION);1994-02
2 Wang Yan Zhang Fengbao Zhu Huaigong Song Zhengxiao Wang Shulan Zeng Peng (School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Fushun Branch, PetroChina Engineering Design Co Ltd. , Fushun 113006, China);Properties of Aminoacylase Immobilized on Macroporous Resin[J];Chemical Reaction Engineering and Technology;2005-01
3 Zhu Xiangrui Xu Junliang Luo Pengfei (Department of Sericulture, Zhejiang University, Hangzhou 310029);Studies on Preparing and Characteristics of Immobilized Catalase in Silk Fibroin[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-03
4 ZHANG Kun-sheng, TIAN Hui-lin (Department of Food Engineering, Tianjin University of Commerce, Tianjin 300134);Research and function of catalase in organism[J];Food Science and Technology;2007-01
5 LI Cheng-xuan FAN Xue-rong WANG Qiang WANG Qin-qing (Key Laboratory of Science and Technology of Eco-Textile, Southern Yangtze University, Wuxi 214122, China);Immobilization of catalase on oxidized cotton fabric[J];Dyeing & Finishing;2007-21
6 WANG Rui-fang CHEN Shan-shan CHOU Nong-xue(Department of Food Engineering,Shanxi Normal University,Xi an 710062);The experimental study of pectinase immobilization by S-8 macroporous absorbent resin[J];China Food Additives;2006-04
Chinese Journal Full-text Database 10 Hits
1 Nan zhirun fan yuexian(Department of art and science,shanxi agriculture of university,taigu 030801);Advance of Researches on Catalase in Plants[J];Anhui Agricultural Science Bulletin;2008-05
2 WANG Fu-ke (Physiology Department of the Basic Science School in Ningxia Medical College,Yinchuan,Ningxia 750004);Research on the α-Amylase Immobilization[J];Journal of Anhui Agricultural Sciences;2008-21
3 QIAN Yi et al(School of Environment and safety Engineering,Qindao University of Science and Technology,Qindao,Shandong 266042);Effects of Cadmium Pollution in soil on Physiological and Biochemical Index of Allium sativum L.[J];Journal of Anhui Agricultural Sciences;2009-18
4 ZHOU Na-lei1,LI Yue1,GAO Yuan2*,CHEN Yong-chun2,YAO min2(1.Clinical Medical Specialized Undergraduate and Postgraduate Class of Hebei Medical University;2.Biochemistry Department of Hebei Medical University,Shijiazhuang 050017,China);Purification and Some Properties of Catalase from Rat Liver[J];Amino Acids & Biotic Resources;2009-02
5 Gu Jinwei Yang Xinlin Zhu Hesun (Research Center of Materials Science,Beijing Institute of Technology,Beijing 100081);Progress in Research on Silk Fibroin as a Kind of Biomaterials[J];Materials Review;2001-11
6 Zhu Xiangrui Xu Junliang(Department of Sericulture and Apiculture,College of Animal Sciences,Zhejiang University,Hangzhou 310029);Preparation and Properties of Immobilization of Glucose Isomerase on Silk Fibroin[J];Acta Sericologica Sinica;2002-03
7 JI Hong,CAO Fu-yu,ZHOU Ji-shu(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100);Preliminary Study on the Effects of Baicalein on CAT,GST and MDA in Hepatocytes and Hepatopancrease of Cyprinus carpio[J];Freshwater Fisheries;2008-03
8 MA Xiu-ling,HUANG Li-mei,CHEN Sheng(College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350007,China);Enzymic Properties of Magnetic Horseradish Peroxidase[J];Journal of Fujian Normal University(Natural Science);2005-04
9 ZHONG Yi-hui1, 2, HUANG Qi-lin1, 2, ZHANG Xin1, HUANG Zhang-jie2, HU Qiu-feng2, YANG Guang-yu21. Department of Chemistry, Yuxi Teacher’s College, Yuxi 653100, China2. Department of Chemistry, Yunnan University, Kunming 650091, China;Study on Solid Phase Extraction and Spectrophotometric Determination of Palladium with MCI-GEL Resin[J];Spectroscopy and Spectral Analysis;2007-02
10 Li Yaowen Liu Shenkui Stress Molecular Biology Laboratory, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, 150040;Isoenzyme of Catalase(CAT) in Rice in Response to Stresses and the Enzymes Activity[J];Genomics and Applied Biology;2009-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 TAN Xing-he 1 ,GAN Lin 2 ,LI Da-zhi 2 ,LI Qing-ming 1 ,GUO SHi-yin 1 ,WANG F eng 1 (1.College of Food Science and Technology,HNAU,Changsha410128,China;2.College of Horticulture and Gardening,HNAU,Changsha410128,China);Application of Enzyme in Processing of Fruit and Ist Fixation Technique[J];Storage and Process;2003-06
2 Lin Yaohui;Liu Xinmin;Shen Yufen;Chen Yiliang;Chen Songhe(Fujian Institute of Subtropical Botany,Xiamen,Fujian,361006);Researches on Enzyme Properties of the Immobilized Pectinase[J];JOURNAL OF FUZHOU UNIVERSITY(NATURAL SCIENCES EDTION);1996-06
3 NIE Hua-li,ZHU Li-min(Institute of Biological Sciences & Biotechnology,Donghua University,Shanghai 200051,China);Fiber as support for immobilized enzyme and its application[J];Journal of Textile Research;2006-07
4 XU Yun-hui, LIN Hong, CHEN Yu-yue (School of Material Engineering, Soochow University, Suzhou,Jiangsu 215021, China);Aggregated structure of cotton fiber oxidised selectively with sodium periodate[J];Journal of Textile Research;2006-11
5 KONG De Ling *, JIA Yong Hui, YU Yao Ting, TONG Ming Rong (Institute for Molecular Biology, Bioactive Materials Research Laboratory, Nankai University, Tianjin, 300071) [JZ〗WONG Tze Fei (Department of Biochemistry, Hong Kong University of S;Oxygen Carrier Based on Immobilized Hemoglobin(Ⅱ) Hemoglobin Covalently Bond to Periodate Oxycellulose[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1998-10
6 JIANG Houli; SONG Zhanjun and LIU Bingyu (National Center of Biomedical Analysis, 100850 Beijing)LIU Changling and SUN Manji(Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,100850 Beijing);DETERMINATION OF THE SECONDARY STRUCTURE OF THREE CATALASES USING THE PARTIAL LEAST-SQUARES INFRARED METHOD[J];SPECTROSCOPY AND SPECTRAL ANALYSIS;1996-02
7 Zheng Wanling, Zhou Yunzhen, Zang Xiangying (Guangdong Institute of Microbiology, Guangzhou 510070);Preparation of Immobilized Enzyme by Strengthened Na alginate Agglutination[J];INDUSTRIAL MICROBIOLOGY;1998-03
8 LIU Xin xi 1,2 , PENG Li feng 2 (1.Editorial Department,Hebei Normal University,Hebei,Shijiazhuang 050091,China; 2.Department of Chemistry,Hebei Normal University,Hebei,Shijiazhuang 050016,China);Studies on Immobilization of Lipase on Cellulose Membrane Using Sodium Periodate Method[J];;2001-01
9 Xu Jingbo Lang Peizhen(Department of EnvironmentaJ Sciences, Northeastern Normal University. Changchun, 130024);THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION OF CATALASE[J];Environmental Chemistry;1994-03
10 YuanKe;LiuYanze;WangLing;WangZhonghui;,JiChunru;WuLingli(Henan College of Traditional Chinese Medicine,Zhengzhou 450003,China)TahgshiYoshida; Takuo Okuda(Okayama University,Okayama 700,Japan);ISOLATION AND CHARACTERIZATION OF TANNIN AND RELATED POLYPHENOLSFROM THE RHIZOME OF RODGERSIA AESCULIFOLIA BATAL[J];Henan Science;1994-01
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