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《Materials Review》 2009-18
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Study on the Immobilization of Catalase by AB-8 Macroporous Absorbent Resin

XIE Xuefeng,ZHANG Zhaohui,CHEN Peice(College of Biotechnology and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014)  
Condition optimization and stability for immobilization of catalase by AB-8 macroporous absorbent resin and glutaraldehyde have been studied.The experimental results indicate that the optimal conditions of catalase immobilization are:the appropriate temperature is 35℃,pH value is 7.0,adsorbent time is 6h,quantity of catalase is 970U per gram carrier,crosslinking time is 2h and the concentration of the crosslinking agent(glutaraldehyde) is 0.2vol%.The maximum yield of immobilized enzyme is 45.2% while catalase is immobilized on those conditions.Compare with the free enzyme,the immobilized enzyme has got certain improvement on thermal stability and pH stability,the remaining activity is more than 60% of the primary enzymeactivity after the immobilized enzyme is used repeatedly for 10 times.It show that the immobilized catalase have good operational stability.
【Fund】: 浙江省科技厅重点计划项目(2006C21117)
【CateGory Index】: TB302
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