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《Chongqing Medical Journal》 2002-01
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Influence of antisense oligodeoxynucleotide targeting against telomerase on expression of carcinoma gene of laryngeal carcinoma cells

PU Yi ping,WU Jia lian,CAO Qian, et al (Department of ENT, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010)  
Objective To study the inhibition of telomerase activity and the influence of expression of carcinoma gene to laryngeal cacinoma cells incubated with AS ODN targeting against telomerase in vitro.Methods Telomerase activity of laryngeal carcinoma cells Hep 2 was analysised by PCR ELISA technique.Expression of P53 and PCNA was detected by immunohistochemical method.Results The AS ODN inhibited telomerase activity of laryngeal carcinoma cells.This procedure is sequential specific and density dependence. The 40μM AS ODN inhibited the expression of PCNA, while had no significant effect on expression of P53 in laryngeal cacinoma cells.Conclusion The AS ODN targeting against telomerase inhibited the expression of PCNA gene in laryngeal carcinoma cells.
【Key Words】: laryngeal carcinoma antisense oligodeoxynucleotide telomerase carcinoma gene
【CateGory Index】: R739.65
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