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《Chongqing Medical Journal》 2004-12
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Mouse model of sclerotic skin induced by local injections of bleomycin

LI Zhi, YAN Guo-fu, HU Mei-ling,et al. (Department of Dermatology,Xinqiao Hospital,the Third Military Medical University,Chongqing 400037,China)  
Objective To establish animal model for scleroderma.Methods Bleomycin was subcutaneously injected into the shaved back skin of female BALB/c mice aged 6 weeks at a dosage of 0.01mg-1mg per day for 4 weeks. The skin change was observed by light and transmission electron microscopy. The expression of TGFβ 1 and a-sma TGFβ 2,was investigated by immunohistochemical staining. Results More than 0.1mg/d of bleomycin could induce dermal sclerosis on mice back skin after 4 weeks. Sclerotic skin with reduced elasticity and hairloss exhibited atrophy of epidermis, inflammatory infiltration in dermis, reduction or disappearance of hair follicles, fibroproliferation with a large number of myofibroblasts, and increased expression of TGFβ 1,TGFβ 2 and a-sma. Conclusion A mouse model for scleroderma can be successfully established in BALB/c mice after 4-week injection of bleomycin.
【Fund】: 国家自然科学基金课题 (30 1 0 0 1 62 )
【CateGory Index】: R-332
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