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《Chongqing Medical Journal》 2005-01
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The study of N-acetylcysteine-restrained apoptosis of human hepatocyte in vitro

WU Gang, YAN Ge, WU Guo-yi,et al. (Institute for Viral Hepatitis, Chongqing University of Medical Sciences,Chongqing 400010,China)  
Objectives To investigate the effects of lipopolysaccharide(LPS) and N-acetylcysteine(NAC) on LO 2 cells in vitro.Medthods The LO 2 cells were cultured for 24h in vitro before the fresh culturing medium with LPS(20μg/ml) or/and NAC were added. DNA-fragment assay was analyzed by the agarose gel electrophoresis to determine apoptostic level. Transmission electron microscopy techniques were used to observe the morphological changes of hepatocyte treated with LPS. The apoptosis of LO 2 cells detected by terminal deoxynucleotide transferas-mediated aUTP nick end labeling(TUNEL). To investigate the restrainable effects of N-acetylcysteine(NAC) .Results LO 2 cells were treated with LPS exhibited some typical apoptosis-specific morphological features. The LPS-induced apoptosis cells decreased when the culture medium contained with LPS and NAC were added for 16h.Conclusions NAC can restrain LPS-induced apoptosis of hepatocyte in vitro.
【CateGory Index】: R575
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