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《Chongqing Medicine》 2006-04
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Modification of suture methods for rat model of focal cerebral ischemia/reperfusion

GONG Biao,LI Chang-qing,HUANG Jian(1.TCM College Chongqing University of Medical Sciences,Chongqing 400050,China;2.Second Hospital Affiliated to Chongqing University of Medical Sciences,Chongqing 400010,China)  
Objective To probe the modification of suture methods for rats model of focal cerebral ischemia/reperfusion.Methods According to the methods of literature,nylon thread was inserted into the internal carotid artery(ICA) in Sprague-Dawley rats to achieve an animal model of reversible middle cerebral artery occlusion.Results While the model was made,the massive haemorrhage while inserting the line that the biggest difficulty met was the bolt thread,and the bolt thread is unable to insert the artery in the neck correctly sometimes.To reduce the bleeding,we tied the command carotid artery(CCA) and ICA loosely with a silk suture besides using vascular forceps.The CCA and the ICA were dissected and divided only when the thread was difficult to be inserted into ICA.Conclusion A silk suture was tied loosely around the CCA and the ICA in advance,which could control bleed effectively conveniently.The CCA and the ICA shoud be reflux only when the thread can not be inserted into ICA correctly and the success rate is obviously raised.
【Fund】: 重庆市科委自然科学资助基金项目(2004-54-83)
【CateGory Index】: R-332
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