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《Chongqing Medicine》 2006-05
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Experimental study of piezoelectric quartz crystal sensor for detecting plasma thrombin time

ZHANG Bo,FU Wei-ling,ZHANG Xue,et al.(Department of Laboratory,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To establish a piezoelectric quartz crystal sensor for determination of the plasma thrombin time(TT).Methods The quartz crystal was 10MHz quartz AT-cut with silver coated electrodes.The oscillating frequency changes were observed in the course of the coagulation reaction of plasma sample,the starting point and the end point of the coagulation reaction were showed by the frequency change tendency.Results(1)After the thrombin was added into the reaction system,oscillation frequency of the crystal was decreased as a ladder,then rose abruptly and descended quickly to balance.The start of the ladder-like frequency decrease was regarded as the start point of coagulation reaction,the turning point of the frequency from rise to decrease as the end point of the reaction.The time between the start point and the end was calculated as TT.(2)The result of statistic analysis showed there was a significant correlation between the results from our method and STAGO method(γ=0.964),the variances within and between batches were 2.26% and 7.53 % respectively.Conclusion Piezoelectric quartz crystal sensor method for determination of the plasma thrombin time is simple,rapid,specific,accurate,cheap,and it might be a valuable method for detection of TT in clinical laboratory.
【Fund】: 国家“863”计划资助项目(2002AARZ2023);; 国家自然科学基金资助项目(20405016);; 国家科技部九五科学仪器攻关项目《科学仪器和实验条件研究与开发》(96-A23-04-04)
【CateGory Index】: R446.11
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Bo,FU Wei ling,YANG Xin,JIANG Tian lun,CHEN Ming,YU Fan (Department of Clinical Laboratories,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Detection of prothrombin time with piezoelectric quartz crystal sensor[J];Acta Academiae Medicine Militaris Tertiae;2002-01
2 Zhang Bo Fu Weiling (Department of Laboratory, Southwest Hospital , Third Military Medical University, Chongqing 400038,China);Advances in Piezoelectric Quartz Crystal Biosensor and Its Applications[J];Journal of Biomedical Engineering;2004-02
3 ZHANG Bo,FU Wei-ling,MAO Qiong-guo,ZHANG Xue, CHEN Ming, JIANG Tian-lun, YU Fan. Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;Study on piezoelectric quartz crystal immunosensor for determining prostate specific antigen[J];Chinese Journal of Laboratory Medicine;2003-07
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